Hemmatpour Hamoon, Haddadi-Asl Vahid, Burgers Thomas C Q, Yan Feng, Stuart Marc C A, Reker-Smit Catharina, Vlijm Rifka, Salvati Anna, Rudolf Petra
Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747AG Groningen, The Netherlands.
Department of Polymer Engineering and Color Technology, Amirkabir University of Technology, P.O. Box 1587-4413, Tehran, Iran.
Nanoscale. 2023 Feb 2;15(5):2402-2416. doi: 10.1039/d2nr06801j.
Administration of temperature-responsive drug carriers that release anticancer drugs at high temperatures can benefit hyperthermia therapies because of the synergistic effect of anticancer drug molecules and high temperature on killing the cancer cells. In this study, we design and characterize a new temperature-responsive nanocarrier based on a naturally occurring and biocompatible clay mineral, halloysite nanotubes. Poly(-isopropylacrylamide) brushes were grown on the surface of halloysite nanotubes using a combination of mussel-inspired dopamine polymerization and surface-initiated atom transfer radical polymerization. The chemical structure of the hybrid materials was investigated using X-ray photoelectron spectroscopy, thermogravimetric analysis and energy-dispersive X-ray spectroscopy. The hybrid material was shown to have a phase transition temperature of about 32 °C, corresponding to a 40 nm thick polymer layer surrounding the nanotubes. Cell studies suggested that grafting of poly(-isopropylacrylamide) brushes on the polydopamine-modified halloysite nanotubes suppresses the cytotoxicity caused by the polydopamine interlayer and drug release studies on nanotubes loaded with doxorubicin showed that thanks to the poly(-isopropylacrylamide) brushes a temperature-dependent drug release is observed. Finally, a fluorescent dye molecule was covalently attached to the polymer-grafted nanotubes and stimulated emission depletion nanoscopy was used to confirm the internalization of the nanotubes in HeLa cells.
给予在高温下释放抗癌药物的温度响应性药物载体可使热疗受益，这是因为抗癌药物分子与高温在杀死癌细胞方面具有协同效应。在本研究中，我们基于天然存在且具有生物相容性的粘土矿物埃洛石纳米管设计并表征了一种新型温度响应性纳米载体。利用受贻贝启发的多巴胺聚合反应与表面引发的原子转移自由基聚合反应相结合的方法，在埃洛石纳米管表面生长聚（N-异丙基丙烯酰胺）刷。使用X射线光电子能谱、热重分析和能量色散X射线光谱对杂化材料的化学结构进行了研究。结果表明，该杂化材料的相变温度约为32℃，对应于围绕纳米管的40nm厚聚合物层。细胞研究表明，在聚多巴胺修饰的埃洛石纳米管上接枝聚（N-异丙基丙烯酰胺）刷可抑制聚多巴胺夹层引起的细胞毒性，并且对负载阿霉素的纳米管进行的药物释放研究表明，由于聚（N-异丙基丙烯酰胺）刷的存在，观察到了温度依赖性药物释放。最后，将荧光染料分子共价连接到聚合物接枝的纳米管上，并使用受激发射损耗纳米显微镜来确认纳米管在HeLa细胞中的内化。
