Xu Xiaoqin, Wang Bin, Zhou Caiquan, Yang Hualin, Zhong Xue, Li Wanhong, Chen Li, Jian Yi, Zhang Long
Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, 637009, Sichuan, China.
Management and Protection Center of Sichuan Fengtongzhai National Nature Reserve, Ya'an, 625700, China.
Mol Biol Rep. 2023 Apr;50(4):3903-3908. doi: 10.1007/s11033-022-08151-0. Epub 2023 Jan 18.
The Chinese monal (Lophophorus lhuysii, Galliformes) is a vulnerable and endemic bird from southwestern China. To better protect this species and increase its population size, genetic markers are urgently needed for investigation and conservation of both wild and captive populations.
By using next-generation sequencing, we developed and characterized markers for seven microsatellite loci of the Chinese monal. PCR examination and statistical analysis indicated that these microsatellites exhibited moderate to high levels of polymorphism, with the expected heterozygosity and polymorphic information content ranging from 0.578 to 0.858 and from 0.540 to 0.841, respectively. Cross-species genome comparison further suggests that these microsatellites are a feature of certain galliform species rather than being specific to the Chinese monal.
A combination of the seven highly polymorphic loci may provide a fundamental genetic toolkit to assess genetic backgrounds and will contribute to design conservation plan, breeding management and other possible studies of the Chinese monal and other evolutionarily related species in the future.
绿尾虹雉（Lophophorus lhuysii，鸡形目）是中国西南部一种易危的特有鸟类。为了更好地保护该物种并增加其种群数量，迫切需要遗传标记来调查和保护野生及圈养种群。
通过使用下一代测序技术，我们开发并鉴定了绿尾虹雉七个微卫星位点的标记。聚合酶链反应（PCR）检测和统计分析表明，这些微卫星表现出中度至高程度的多态性，预期杂合度和多态信息含量分别在0.578至0.858以及0.540至0.841之间。跨物种基因组比较进一步表明，这些微卫星是某些鸡形目物种的特征，而非绿尾虹雉所特有。
这七个高度多态性位点的组合可能提供一个基本的遗传工具包来评估遗传背景，并将有助于未来设计绿尾虹雉及其他进化相关物种的保护计划、育种管理和其他可能的研究。
