二亚乙基三胺-β-环糊精修饰的碳量子点用于 Hg 和 Fe 的选择性荧光传感及细胞成像。
Diethylenetriamine-β-CD-modified carbon quantum dots for selective fluorescence sensing of Hg and Fe and cellular imaging.
机构信息
Key Laboratory of Intelligent Supramolecular Chemistry at the University of Yunnan Province, National and Local Joint Engineering Research Center for Green Preparation Technology of Biobased Materials, School of Chemistry & Environment, Yunnan Minzu University, Kunming 650500, PR China.
Key Laboratory of Intelligent Supramolecular Chemistry at the University of Yunnan Province, National and Local Joint Engineering Research Center for Green Preparation Technology of Biobased Materials, School of Chemistry & Environment, Yunnan Minzu University, Kunming 650500, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Apr 15;291:122364. doi: 10.1016/j.saa.2023.122364. Epub 2023 Jan 12.
Diethylenetriamine-β-cyclodextrin-modified carbon quantum dots (3 N-CQDs) were synthesized via a one-step hydrothermal method using citric acid as the carbon source and diethylenetriamine-β-cyclodextrin (3 N-β-CD) as the nitrogen source. The successful preparation of 3 N-CQDs were revealed by infrared absorption spectroscopy, ultraviolet (UV)-visible absorption spectroscopy, fluorescence spectroscopy, XRD, XPS, TEM, and TG. Further spectroscopic studies showed that the synthesized carbon quantum dots offered good anti-interference capability. The relative fluorescence quantum yield was 67.2 %. The limits of detection for Hg and Fe were 0.25 µM and 0.57 μM, respectively. Cytotoxicity and imaging studies showed that the prepared carbon quantum dots had low cytotoxicity, good biocompatibility, and good cellular imaging capability for HeLa cells. They offered fluorescent sensing of Hg and Fe in live cells. Therefore, 3 N-CQDs were ideal fluorescent probes for the detection of Hg and Fe in water.
二亚乙基三胺-β-环糊精修饰的碳量子点（3N-CQDs）通过一步水热法合成，以柠檬酸为碳源，二亚乙基三胺-β-环糊精（3N-β-CD）为氮源。通过红外吸收光谱、紫外（UV）-可见吸收光谱、荧光光谱、XRD、XPS、TEM 和 TG 揭示了 3N-CQDs 的成功制备。进一步的光谱研究表明，合成的碳量子点具有良好的抗干扰能力。相对荧光量子产率为 67.2%。Hg 和 Fe 的检测限分别为 0.25µM 和 0.57µM。细胞毒性和成像研究表明，所制备的碳量子点具有低细胞毒性、良好的生物相容性和对 HeLa 细胞良好的细胞成像能力。它们可对活细胞中的 Hg 和 Fe 进行荧光传感。因此，3N-CQDs 是用于水中 Hg 和 Fe 检测的理想荧光探针。
Zotero 插件