双斑萤叶甲（鞘翅目：叶甲科）的染色体水平基因组组装

Chromosome-level genome assembly of Monolepta hieroglyphica, two-spotted leaf beetle (Coleoptera: Chrysomelidae).

作者信息

He Hao, Zhao Yongxin, Zheng Dengyu, Kuang Huiyun, Shen Fei, He Kang, Zhang Haiyan, Zhao Changjiang, Jiang Lu, Xiao Da, Wang Su, Wang Zhenying, Zhan Shuai, Wei Jianhua, Yang Xiaozeng, Wu Zhongyi, Zhang Chun

机构信息

Institute of Biotechnology, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.

Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China.

出版信息

Sci Data. 2025 Jan 2;12(1):1. doi: 10.1038/s41597-024-04360-3.

DOI:10.1038/s41597-024-04360-3

PMID:39747111

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11696759/

Abstract

Monolepta hieroglyphica, in view of its wide-ranging host and highly polyphagous characteristics, has become an important agricultural pest in East and Southeast Asian countries. To better understand its biology and develop control strategies, we present a high-quality chromosome-level genome assembly of M. hieroglyphica, with contig N50 of 18.62 Mb and scaffold N50 of 241.916 Mb. Using 156x Hi-C data, we anchored a total length of 2,313 Mb (91.9% of the genome) to nine chromosomes. We predicted 46,561 genes using an integrated approach. The completeness of the final reported genome and proteome were assessed by BUSCO and show high values of 99.4% and 95.2%, respectively. The high-quality chromosome-level genome assembly of M. hieroglyphica will be a useful molecular resource for the communities of both beetle and crop protection.

摘要

鉴于稻黄星步行虫具有广泛的寄主范围和高度多食性的特点，它已成为东亚和东南亚国家一种重要的农业害虫。为了更好地了解其生物学特性并制定防治策略，我们展示了稻黄星步行虫高质量的染色体水平基因组组装结果，其重叠群N50为18.62 Mb，支架N50为241.916 Mb。利用156x Hi-C数据，我们将总长2313 Mb（占基因组的91.9%）定位到9条染色体上。我们采用综合方法预测了46,561个基因。最终报告的基因组和蛋白质组的完整性通过BUSCO评估，分别显示出99.4%和95.2%的高值。稻黄星步行虫高质量的染色体水平基因组组装将为甲虫和作物保护领域的研究群体提供有用的分子资源。