Murakami Shigeru, Hirazawa Chihiro, Mizutani Toshiki, Yoshikawa Rina, Ohya Takuma, Ma Ning, Owaki Yutaka, Owaki Toyohiro, Ito Takashi, Matsuzaki Chiaki
Department of Bioscience and Biotechnology Fukui Prefectural University Fukui Japan.
Fukui Bioincubation Center (FBIC) Fukui Prefectural University Fukui Japan.
Food Sci Nutr. 2022 Oct 17;11(1):599-610. doi: 10.1002/fsn3.3100. eCollection 2023 Jan.
Obesity and diabetes are serious, chronic medical conditions associated with a wide range of life-threatening conditions. The aim of this study was to investigate the effects of the edible red seaweed (Postels et Ruprecht) J. Agardh () on the development of obesity, diabetes and related metabolic diseases in mice. Male C57BL/6J mice were fed a high-fat (HF) diet (60% energy as fat), or an HF diet containing 2% (w/w) or 6% powdered for 13 weeks. Polysaccharides of were isolated and their anti-inflammatory effects were evaluated in lipopolysaccharide-stimulated RAW264.7 cells. The HF diet group showed greater weight gain, lipid accumulation in the body and liver, and increased serum levels of glucose and cholesterol in comparison to the normal group fed a normal diet (10% energy as fat). The treatment of HF diet mice with reduced these changes and stimulated the fecal excretion of fat. In addition, suppressed the HF diet-induced elevation of inflammation and oxidative stress markers in the serum and liver. The isolated sulfated polysaccharide from inhibited pancreatic lipase activity and decreased the production of nitric oxide and TNF-α in the murine macrophage cell line RAW264.7. These results show that treatment can attenuate obesity, diabetes, hepatic steatosis, and dyslipidemia in mice fed an HF diet, which is associated with inhibited intestinal fat absorption and reduced inflammation and oxidative stress by a sulfated polysaccharide.
肥胖和糖尿病是严重的慢性疾病，与多种危及生命的状况相关。本研究的目的是调查可食用红藻（Postels et Ruprecht）J. Agardh（）对小鼠肥胖、糖尿病及相关代谢疾病发展的影响。雄性C57BL/6J小鼠被喂食高脂（HF）饮食（60%的能量来自脂肪），或含有2%（w/w）或6% 粉末的HF饮食，持续13周。分离出 的多糖，并在脂多糖刺激的RAW264.7细胞中评估其抗炎作用。与喂食正常饮食（10%的能量来自脂肪）的正常组相比，HF饮食组体重增加更多，体内和肝脏脂质积累更多，血清葡萄糖和胆固醇水平升高。用 处理HF饮食小鼠可减少这些变化，并刺激脂肪的粪便排泄。此外， 抑制了HF饮食诱导的血清和肝脏中炎症和氧化应激标志物的升高。从 中分离出的硫酸化多糖抑制了胰腺脂肪酶活性，并减少了小鼠巨噬细胞系RAW264.7中一氧化氮和TNF-α的产生。这些结果表明， 处理可减轻喂食HF饮食小鼠的肥胖、糖尿病、肝脂肪变性和血脂异常，这与硫酸化多糖抑制肠道脂肪吸收、减轻炎症和氧化应激有关。
