催化活性包涵体的设计、生产与表征

Design, Production, and Characterization of Catalytically Active Inclusion Bodies.

作者信息

Ölçücü Gizem, Jaeger Karl-Erich, Krauss Ulrich

机构信息

Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich, Wilhelm-Johnen-Straße, Jülich, Germany.

Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Wilhelm-Johnen-Straße, Jülich, Germany.

出版信息

Methods Mol Biol. 2023;2617:49-74. doi: 10.1007/978-1-0716-2930-7_4.

DOI:10.1007/978-1-0716-2930-7_4

PMID:36656516

Abstract

Catalytically active inclusion bodies (CatIBs) are promising biologically produced enzyme/protein immobilizates for application in biocatalysis, synthetic chemistry, and biomedicine. CatIB formation is commonly induced by fusion of suitable aggregation-inducing tags to a given target protein. Heterologous production of the fusion protein in turn yields CatIBs. This chapter presents the methodology needed to design, produce, and characterize CatIBs.

摘要

具有催化活性的包涵体（CatIBs）是有望通过生物方法生产的酶/蛋白质固定化产物，可应用于生物催化、合成化学和生物医学领域。CatIB的形成通常是通过将合适的聚集诱导标签与特定目标蛋白融合来诱导的。融合蛋白的异源生产进而产生CatIBs。本章介绍了设计、生产和表征CatIBs所需的方法。