Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, USA.
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, USA; United States Department of Agriculture, Agricultural Research Service, Animal Disease Research Unit, Pullman, WA, 99164, USA.
Comp Immunol Microbiol Infect Dis. 2021 Jun;76:101641. doi: 10.1016/j.cimid.2021.101641. Epub 2021 Mar 3.
Mycoplasma ovipneumoniae is a respiratory pathogen that impacts domestic sheep (Ovis aries; DS) and bighorn sheep (Ovis canadensis; BHS). BHS are reported to be more susceptible than DS to developing polymicrobial pneumonia associated with M. ovipneumoniae infection. Using formalin-fixed paraffin-embedded tissues, we performed a retrospective study investigating the pulmonary immune response of DS and BHS to M. ovipneumoniae infection. M. ovipneumoniae infected DS exhibited a more robust and well-organized BALT formation as compared to BHS. Digital analysis of immunohistochemical chromogen deposition in lung tissue was used to quantitate T cell marker CD3, B cell markers CD20 and CD79a, macrophage markers CD163 and Iba1, and cytokine IL-17. A significant interaction of species and infection status was identified for CD3, CD163, and IL-17. BHS had a greater increase in bronchiolar CD3 and bronchiolar and alveolar CD163 with infection, as compared to DS. BHS had an increase in bronchiolar associated lymph tissue (BALT) and alveolar IL-17 with infection, while these remained similar in DS regardless of infection status. IL-17 in respiratory epithelium of bronchi and bronchioles comparatively decreased in DS and increased in BHS with infection. These data begin to define the interspecies differential immune response to pulmonary M. ovipneumoniae infection in DS and BHS and provide the first investigations of respiratory epithelium-associated IL-17 in ovine.
绵羊肺炎支原体是一种呼吸道病原体，可感染绵羊（Ovis aries；DS）和大角羊（Ovis canadensis；BHS）。据报道，BHS 比 DS 更容易感染绵羊肺炎支原体感染引起的多微生物肺炎。我们使用福尔马林固定石蜡包埋组织，进行了一项回顾性研究，以调查 DS 和 BHS 对绵羊肺炎支原体感染的肺部免疫反应。与 BHS 相比，感染绵羊肺炎支原体的 DS 表现出更强大和组织良好的支气管相关淋巴组织（BALT）形成。对肺组织免疫组织化学显色剂沉积的数字分析用于定量 T 细胞标志物 CD3、B 细胞标志物 CD20 和 CD79a、巨噬细胞标志物 CD163 和 Iba1 以及细胞因子 IL-17。物种和感染状态的显著相互作用被确定为 CD3、CD163 和 IL-17。与 DS 相比，感染后 BHS 的支气管 CD3 和支气管和肺泡 CD163 增加更大。BHS 的感染导致支气管相关淋巴组织（BALT）和肺泡 IL-17 增加，而 DS 无论感染状态如何，这些都保持相似。感染后，DS 的支气管和细支气管呼吸上皮中的 IL-17 相对减少，而 BHS 中的 IL-17 增加。这些数据开始定义 DS 和 BHS 对肺绵羊肺炎支原体感染的种间差异免疫反应，并首次研究了绵羊的呼吸上皮细胞相关的 IL-17。
