Yin Lijuan, Li Yaru, Zhang Wenlu, Han Xiao, Wu Qiankun, Xie Yanyan, Fan Jingjing, Ma Long
State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, National and Local United Engineering Lab of Metabolic Control Fermentation Technology, China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Academy of National Food and Strategic Reserves Administration, Beijing, 100037, China.
J Agric Food Chem. 2023 Mar 1;71(8):3551-3563. doi: 10.1021/acs.jafc.2c06537. Epub 2023 Jan 19.
Foodborne viruses have been recognized as important threats to food safety and human health. Rapid and accurate detection is one of the most crucial measures for food safety control. With the development of biology, chemistry, nanoscience, and related interdisciplines, detection strategies have been devised and advanced continuously. This review mainly focuses on the progress of detection methods for foodborne viruses. The current detection methods for foodborne viruses are summarized, including traditional electron microscopy and cultural isolation, immunoassay, molecular technology, biosensors, and newly emerging CRISPR/Cas-based detection technology. Furthermore, a comparison of the detection methods was objectively discussed. This review provides a comprehensive account of foodborne virus detection methods from fundamentals to state-of-the-art and illustrates the advantages and disadvantages of the current methods and proposes the future trends and directions for foodborne virus detection. It is hoped that this review can update current knowledge and present blueprints in order to accelerate futuristic development.
食源性病毒已被公认为食品安全和人类健康的重要威胁。快速准确的检测是食品安全控制的关键措施之一。随着生物学、化学、纳米科学及相关交叉学科的发展，检测策略不断被设计和推进。本综述主要聚焦于食源性病毒检测方法的进展。总结了目前食源性病毒的检测方法，包括传统的电子显微镜和培养分离、免疫测定、分子技术、生物传感器以及新兴的基于CRISPR/Cas的检测技术。此外，还客观地讨论了各检测方法之间的比较。本综述全面介绍了从基础到最新技术的食源性病毒检测方法，阐述了当前方法的优缺点，并提出了食源性病毒检测的未来趋势和方向。希望本综述能够更新现有知识并提供蓝图，以加速未来的发展。
