Mills J S, Needham M, Thompson T C, Parker M G
Molecular Endocrinology Laboratory, Imperial Cancer Research Fund, London, U.K.
Mol Cell Endocrinol. 1987 Sep;53(1-2):111-8. doi: 10.1016/0303-7207(87)90197-3.
Two proteins of molecular weights 25 and 12 kDa (p25 and p12 respectively), whose expression is regulated by testosterone, were identified in mouse ventral prostate. An antiserum raised to mouse ventral prostate secretion was used to demonstrate that p25 corresponds to the major secretory glycoprotein in mouse prostatic fluid. This antiserum does not cross-react with the major secretory proteins of the rat ventral prostate. Western blot analysis of mouse ventral prostate proteins using the prostatic secretion antiserum demonstrates that p12 and p25 are detectable at 3 weeks of age, but the maximum level of both proteins is not attained until 5 weeks of age. In addition, synthesis of p25 was also observed in prostate tissue derived from differentiated embryonic urogenital sinus tissue growing as implants under the renal capsule of syngeneic male hosts.
在小鼠腹侧前列腺中鉴定出两种分子量分别为25 kDa和12 kDa的蛋白质（分别为p25和p12），其表达受睾酮调节。用针对小鼠腹侧前列腺分泌物产生的抗血清来证明p25对应于小鼠前列腺液中的主要分泌糖蛋白。该抗血清与大鼠腹侧前列腺的主要分泌蛋白无交叉反应。用前列腺分泌抗血清对小鼠腹侧前列腺蛋白进行蛋白质免疫印迹分析表明，在3周龄时可检测到p12和p25，但直到5周龄时这两种蛋白质才达到最高水平。此外，在同基因雄性宿主肾被膜下作为移植物生长的分化胚胎泌尿生殖窦组织来源的前列腺组织中也观察到了p25的合成。
