Norman J T, Cunha G R, Sugimura Y
Prostate. 1986;8(3):209-20. doi: 10.1002/pros.2990080302.
Tissue recombinants were prepared with a single epithelial ductal tip from adult prostate and mesenchyme from either the embryonic urogenital sinus or adult urinary bladder. Recombinants were grown in vivo beneath the renal capsule of male hosts. After 4 weeks of in vivo growth, extensive growth of arborizing ducts was apparent in recombinants composed of urogenital sinus mesenchyme and a single adult prostatic ductal tip. One-dimensional polyacrylamide gel electrophoresis indicated that these recombinants contained many of the proteins of the mature prostate. Heterospecific recombinants (rat urogenital sinus mesenchyme and mouse prostatic epithelium) showed the ductal tissue to be derived solely from the prostatic epithelium. In recombinants of a prostatic ductal tip with mesenchyme from the urinary bladder, ductal growth was absent, the ductal tip was maintained as a single, discrete, epithelial structure, and the protein composition of these recombinants more closely resembled that of the bladder. The results demonstrate that the epithelial cells of the adult prostate can participate in new ductal growth in response to an embryonic prostatic inductor. These data provide experimental evidence to support the hypothesis that human benign prostatic hyperplasia may result from the anomalous reactivation of embryonic growth potential in the adult prostate.
用成年前列腺的单个上皮导管尖端与来自胚胎泌尿生殖窦或成年膀胱的间充质制备组织重组体。重组体在雄性宿主的肾包膜下进行体内培养。经过4周的体内生长，在由泌尿生殖窦间充质和单个成年前列腺导管尖端组成的重组体中，明显可见分支导管的广泛生长。一维聚丙烯酰胺凝胶电泳表明，这些重组体含有许多成熟前列腺的蛋白质。异种特异性重组体（大鼠泌尿生殖窦间充质和小鼠前列腺上皮）显示导管组织仅来源于前列腺上皮。在前列腺导管尖端与膀胱间充质的重组体中，没有导管生长，导管尖端保持为单个离散的上皮结构，并且这些重组体的蛋白质组成更类似于膀胱。结果表明，成年前列腺的上皮细胞可响应胚胎前列腺诱导物参与新的导管生长。这些数据提供了实验证据来支持这样的假说，即人类良性前列腺增生可能是由于成年前列腺中胚胎生长潜能的异常重新激活所致。
