Kim Yu-Jin, Nitin Nitin, Kim Kyu-Bong
College of Pharmacy, Dankook University, 119 Dandae-ro, Cheonan 31116, Chungnam, Republic of Korea.
Center for Human Risk Assessment, Dankook University, Cheonan 31116, Chungnam, Republic of Korea.
Toxics. 2023 Jan 11;11(1):67. doi: 10.3390/toxics11010067.
Glyphosate is a non-selective herbicide. Although glyphosate is not acutely toxic, the intake of glyphosate-based herbicides has caused many accidents. Some studies have suggested that surfactants might be the cause. The purpose of this study was to compare the toxicokinetic (TK) properties of glyphosate according to different vehicles in rats. Glyphosate (1%) was dissolved in distilled water (DW), polyoxyethylene tallow amine (POEA), and Tween 20. After a single oral treatment of glyphosate (50 mg/kg), blood was collected at time intervals, and glyphosate concentrations in the target organ (liver and kidney) were determined 24 h after final blood collection. All samples were analyzed using LC-MS/MS. The TK parameters of glyphosate were similar in the DW and Tween 20 groups. However, there were significant differences in Tmax and volume of distribution (Vd) between the DW and POEA group (p < 0.05). Glyphosate was absorbed about 10 times faster in POEA group rather than DW, and exhibited a higher distribution. However, other important TK parameters of T1/2, AUC, and Cmax were not statistically different among the different vehicle groups. Although glyphosate concentration in the liver was significantly higher in the POEA group than in the DW group, there was no significant difference in the kidney. These results indicate that the toxicokinetics of glyphosate are not significantly affected by POEA. It can be concluded that POEA toxicity itself can be attributed to the acute toxicity of glyphosate-containing products.
草甘膦是一种非选择性除草剂。尽管草甘膦没有急性毒性，但摄入基于草甘膦的除草剂已导致许多事故。一些研究表明表面活性剂可能是原因。本研究的目的是比较大鼠中草甘膦在不同载体下的毒代动力学（TK）特性。将草甘膦（1%）溶解于蒸馏水（DW）、聚氧乙烯牛脂胺（POEA）和吐温20中。单次口服草甘膦（50mg/kg）后，按时间间隔采集血液，并在最后一次采血后24小时测定靶器官（肝脏和肾脏）中的草甘膦浓度。所有样品均使用LC-MS/MS进行分析。草甘膦在DW组和吐温20组中的TK参数相似。然而，DW组和POEA组之间的达峰时间（Tmax）和分布容积（Vd）存在显著差异（p<0.05）。草甘膦在POEA组中的吸收速度比DW组快约10倍，且分布更高。然而，不同载体组之间的其他重要TK参数，如半衰期（T1/2）、曲线下面积（AUC）和峰浓度（Cmax）在统计学上没有差异。尽管POEA组肝脏中的草甘膦浓度显著高于DW组，但肾脏中没有显著差异。这些结果表明草甘膦的毒代动力学不受POEA的显著影响。可以得出结论，POEA的毒性本身可归因于含草甘膦产品的急性毒性。
