Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
Key Laboratory of Consumer Product Quality Safety Inspection and Risk Assessment for State Market Regulation, Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
Biosensors (Basel). 2023 Jan 13;13(1):133. doi: 10.3390/bios13010133.
In general, co-reactants are essential in highly efficient electrochemiluminescence (ECL) systems. Traditional co-reactants are usually toxic, so it is necessary to develop new environmentally friendly co-reactants. In this work, carbon dots (CDs) were assembled with dendritic silica nanospheres (CDs@dSiO NPs) to form a co-reactant of Ru(bpy). Subsequently, a sandwich immunosensor for detecting human chorionic gonadotropin (HCG) was constructed based on CDs@dSiO NPs as co-reactants, the nanoprobe loaded with the secondary antibody, and Ru(bpy) as a luminophore. In addition, compared to directly as a signal probe, the luminophore Ru (bpy) as a part of the electrolyte solution is simpler in this work. The immunosensor has an extremely low limit of detection of 0.00019 mIU/mL. This work describes the synthesis of low-toxic, efficient, and environmentally friendly CDs, which have become ideal co-reactants of Ru(bpy), and proposes an ECL immunosensor with excellent stability and selectivity, which has great potential in clinical applications.
一般来说，共反应物在高效电致化学发光（ECL）系统中是必不可少的。传统的共反应物通常是有毒的，因此有必要开发新的环保共反应物。在这项工作中，碳点（CDs）与树枝状二氧化硅纳米球（CDs@dSiO NPs）组装在一起，形成 Ru(bpy)的共反应物。随后，基于 CDs@dSiO NPs 作为共反应物、负载第二抗体的纳米探针以及 Ru(bpy)作为发光体，构建了用于检测人绒毛膜促性腺激素（HCG）的三明治免疫传感器。此外，与直接作为信号探针相比，发光体 Ru(bpy)作为电解质溶液的一部分在这项工作中更为简单。该免疫传感器的检测限极低，为 0.00019 mIU/mL。本工作描述了低毒、高效、环保的 CDs 的合成，它们已成为 Ru(bpy)的理想共反应物，并提出了一种具有优异稳定性和选择性的 ECL 免疫传感器，在临床应用中具有巨大的潜力。
