State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Genes (Basel). 2022 Dec 28;14(1):94. doi: 10.3390/genes14010094.
miRNAs are a set of small, noncoding RNAs that bind to partially complementary sequences on target mRNAs. This leads to the post-transcriptional regulation of gene expression. Many studies have shown that microRNAs play critical roles in adipose cell differentiation and fat metabolism. The aim of this study was to explore the regulatory functions of miRNAs in fat deposition for the prevention and therapy of lipid metabolism-related diseases.
The significant differences in the fat deposition of Laiwu (LW) pigs and Large White (LY) pigs were studied. To investigate the genetic relationships of miRNAs that regulate fat deposition, we performed a genome-wide analysis of miRNAs derived from subcutaneous adipose tissue of LW and LY pigs using RNA-seq.
There were 39 known miRNAs and 56 novel miRNAs significantly differential expressed between the two breeds of pigs. In the analysis of the Gene Ontology and KEGG pathways, predicted targets of these differentially expressed miRNAs were involved in several fat-associated pathways, such as the peroxisome proliferator-activated receptor (PPAR), mitogen-activated protein kinases (MAPK) and Wnt signaling pathways. In addition, ssc-miR-133a-3p, ssc-miR-486 and ssc-miR-1 each had a great impact on the development of porcine subcutaneous fat through the PPAR signaling pathway.
We explored the role of differentially expressed miRNAs and studied the mechanisms of adipogenesis and fat deposition between two different pig breeds. In addition, these results also contribute to research relevant to human obesity.
miRNAs 是一组小的非编码 RNA，可与靶 mRNA 上部分互补的序列结合。这导致基因表达的转录后调控。许多研究表明，microRNAs 在脂肪细胞分化和脂肪代谢中发挥关键作用。本研究旨在探讨 miRNAs 在脂肪沉积中的调节功能，以预防和治疗与脂质代谢相关的疾病。
研究了莱芜猪和大白猪脂肪沉积的显著差异。为了研究调节脂肪沉积的 miRNAs 的遗传关系，我们使用 RNA-seq 对莱芜猪和大白猪皮下脂肪组织中的 miRNAs 进行了全基因组分析。
两个品种猪之间有 39 个已知的 miRNAs 和 56 个新的 miRNAs 表达显著差异。在基因本体论和 KEGG 通路分析中，这些差异表达的 miRNAs 的预测靶基因参与了几个与脂肪相关的途径，如过氧化物酶体增殖物激活受体 (PPAR)、丝裂原激活蛋白激酶 (MAPK) 和 Wnt 信号通路。此外，ssc-miR-133a-3p、ssc-miR-486 和 ssc-miR-1 均通过 PPAR 信号通路对猪皮下脂肪的发育有很大影响。
我们探讨了差异表达 miRNAs 的作用，并研究了两个不同猪品种之间脂肪生成和脂肪沉积的机制。此外，这些结果也有助于研究与人类肥胖相关的问题。
