Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Sci Rep. 2016 Nov 29;6:37983. doi: 10.1038/srep37983.
Goat fecundity is important for agriculture and varies depending on the genetic background of the goat. Two excellent domestic breeds in China, the Jining Grey and Laiwu Black goats, have different fecundity and prolificacies. To explore the potential miRNAs that regulate the expression of the genes involved in these prolific differences and to potentially discover new miRNAs, we performed a genome-wide analysis of the miRNAs in the ovaries from these two goats using RNA-Seq technology. Thirty miRNAs were differentially expressed between the Jining Grey and Laiwu Black goats. Gene Ontology and KEGG pathway analyses revealed that the target genes of the differentially expressed miRNAs were significantly enriched in several biological processes and pathways. A protein-protein interaction analysis indicated that the miRNAs and their target genes were related to the reproduction complex regulation network. The differential miRNA expression profiles found in the ovaries between the two distinctive breeds of goats studied here provide a unique resource for addressing fecundity differences in goats.
山羊繁殖力对农业很重要，其受山羊的遗传背景影响。中国有两个优秀的国内品种，济宁青山羊和莱芜黑山羊，它们具有不同的繁殖力和多产性。为了探讨可能调节这些多产差异相关基因表达的 miRNA，我们使用 RNA-Seq 技术对这两种山羊的卵巢中的 miRNA 进行了全基因组分析。在济宁青山羊和莱芜黑山羊之间有 30 个 miRNA 存在差异表达。GO 和 KEGG 通路分析表明，差异表达 miRNA 的靶基因在几个生物学过程和通路中显著富集。蛋白质-蛋白质相互作用分析表明，miRNAs 和它们的靶基因与生殖复合物的调控网络有关。本研究中在两个不同品种的山羊卵巢中发现的差异 miRNA 表达谱为研究山羊的繁殖力差异提供了独特的资源。
