Piseskul Jirachaya, Suttisansanee Uthaiwan, Chupeerach Chaowanee, Khemthong Chanakan, Thangsiri Sirinapa, Temviriyanukul Piya, Sahasakul Yuraporn, Santivarangkna Chalat, Chamchan Rungrat, Aursalung Amornrat, On-Nom Nattira
Food and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, Thailand.
Foods. 2023 Jan 8;12(2):292. doi: 10.3390/foods12020292.
subsp. W.J.de Wlide & Duyfjes (or Making in Thai) is a neglected and underutilized crop (NUC) with high fat containing nuts. In this study, the enzyme-assisted mechanical extraction of seed oil was investigated using response surface methodology (RSM) to predict the optimal fat extraction conditions. The most efficient enzyme used in the experiment was a mixture of Flavourzyme and Viscozyme (1:1, /). The predicted maximum oil yield was 46.44%, using the following extraction conditions: 2.98% (/) enzyme loading, 48 °C incubation temperature and 76 min of incubation time. seed oil obtained from heat and enzymatic pretreatments exhibited the highest lightness and viscosity. The chemical properties of this seed oil, including water and volatile compounds (≤0.2% /), acid value (≤4.0 mg KOH/g), peroxide value (≤15 mEq of active oxygen/kg) and soap content (≤0.005% /), were within the acceptable levels specified by the Codex Alimentarius (2019). seed oil obtained from heat and enzymatic pretreatments contained the highest content of δ-tocopherol (88.29 mg/100 g) and omega-6 fatty acids (48.19 g/100 g). This study is the first to report on the enzyme-assisted mechanical extraction of oilseeds as a promising plant material for vegetable oil production.
亚种W.J.de Wlide & Duyfjes（或泰语名称为Making）是一种被忽视且未充分利用的作物（NUC），其坚果富含脂肪。在本研究中，采用响应面法（RSM）研究了酶辅助机械提取种子油，以预测最佳脂肪提取条件。实验中使用的最有效酶是风味酶和粘性酶的混合物（1:1，/）。预测的最大出油率为46.44%，采用以下提取条件：酶负载量2.98%（/）、孵育温度48℃和孵育时间76分钟。经加热和酶预处理获得的种子油具有最高的亮度和粘度。这种种子油的化学性质，包括水分和挥发性化合物（≤0.2% /）、酸值（≤4.0 mg KOH/g）、过氧化值（≤15 mEq活性氧/kg）和皂含量（≤0.005% /），均在食品法典委员会（2019年）规定的可接受水平范围内。经加热和酶预处理获得的种子油中δ-生育酚（88.29 mg/100 g）和ω-6脂肪酸（48.19 g/100 g）的含量最高。本研究首次报道了酶辅助机械提取油籽作为植物油生产有前景的植物材料。
