Synthetic Biology for Clinical and Technological Innovation, National University of Singapore, 14 Medical Drive, Singapore 117599, Singapore.
Synthetic Biology Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119077, Singapore.
Int J Mol Sci. 2023 Jan 9;24(2):1259. doi: 10.3390/ijms24021259.
We report enzymes from the berberine bridge enzyme (BBE) superfamily that catalyze the oxidative cyclization of the monoterpene moiety in cannabigerolic acid (CBGA) to form cannabielsoin (CBE). The enzymes are from a variety of organisms and are previously uncharacterized. Out of 232 homologues chosen from the enzyme superfamily, four orthologues were shown to accept CBGA as a substrate and catalyze the biosynthesis of CBE. The four enzymes discovered in this study were recombinantly expressed and purified in . These enzymes are the first report of heterologous expression of BBEs that did not originate from the plant that catalyze the production of cannabinoids using CBGA as substrate. This study details a new avenue for discovering and producing natural and unnatural cannabinoids.
我们报告了来自小檗碱桥酶（BBE）超家族的酶，它们催化大麻萜酚酸（CBGA）中单萜部分的氧化环化，形成大麻素（CBE）。这些酶来自各种生物体，以前尚未被描述。从酶超家族中选择的 232 个同源物中，有 4 个直系同源物被证明可以接受 CBGA 作为底物，并催化 CBE 的生物合成。本研究中发现的这 4 种酶在. 中被重组表达和纯化。这些酶是首次报道的异源表达 BBE，它们不源自 植物，而是使用 CBGA 作为底物催化大麻素的产生。本研究详细介绍了发现和生产天然和非天然大麻素的新途径。
