Levkut Martin, Levkutová Mária, Grešáková Ľubomíra, Bobíková Katarína, Revajová Viera, Dvorožňáková Emília, Ševčíková Zuzana, Herich Róbert, Karaffová Viera, Žitňan Rudolf, Levkut Mikuláš
Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Department of Epizootiology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovakia.
Life (Basel). 2022 Dec 26;13(1):67. doi: 10.3390/life13010067.
The effect of inorganic zinc and Ascaridia galli infection was studied on MUC1, MUC2 (mucin), sIgA (secretory immunoglobulin A), and metallothionein in the intestines of broilers. Thirty-five-day-old chickens (n = 24), COBB 500 breed, were included in a 14-day experiment. Chickens were divided into 4 groups of 6 chickens each: control ©, Ascaridia galli (AG), Zinc group (Zn), and combined group (AG + Zn). Samples from the intestine for determination of MUC1, MUC2, sIgA, and metallothionein were taken at 7 and 14 days during necropsy. Samples from the jejunum for determination of MUC1, MUC2, sIgA, and metallothionein were taken at 7 and 14 days during necropsy. The results demonstrated that 12 days’ administration of inorganic zinc increased production of MUC1 (p < 0.0001) and MUC2 (p < 0.001) in the Ascaridia galli-infected group (Ag + Zn) in comparison to control (C). The beneficial effect of zinc was also revealed in the production of sIgA (p < 0.0001) in the combined group (AG + Zn) at 7 days. The concentration of metallothionein increased mainly in the zinc group (p < 0.01) of first sampling and was upregulated in Zn and AG + Zn groups. The obtained data indicate the use of inorganic zinc as a suitable immunomodulator of intestinal immunity in Ascaridia galli-infected chickens.
研究了无机锌和鸡蛔虫感染对肉鸡肠道中MUC1、MUC2（黏蛋白）、sIgA（分泌型免疫球蛋白A）和金属硫蛋白的影响。选用35日龄的COBB 500品种鸡（n = 24）进行为期14天的实验。将鸡分为4组，每组6只：对照组（C）、鸡蛔虫感染组（AG）、锌组（Zn）和联合组（AG + Zn）。在尸检时于第7天和第14天采集肠道样本以测定MUC1、MUC2、sIgA和金属硫蛋白。在尸检时于第7天和第14天采集空肠样本以测定MUC1、MUC2、sIgA和金属硫蛋白。结果表明，与对照组（C）相比，在鸡蛔虫感染组（Ag + Zn）中，连续12天给予无机锌可增加MUC1（p < 0.0001）和MUC2（p < 0.001）的产生。在联合组（AG + Zn）中，第7天时sIgA的产生也显示出锌的有益作用（p < 0.0001）。金属硫蛋白的浓度主要在首次采样时的锌组中升高（p < 0.01），并且在锌组和AG + Zn组中上调。所得数据表明无机锌可作为鸡蛔虫感染鸡肠道免疫的合适免疫调节剂。
