Dorm Bruna Carolina, Iemma Mônica Rosas Costa, Neto Benedito Domingos, Francisco Rauany Cristina Lopes, Dinić Ivana, Ignjatović Nenad, Marković Smilja, Vuković Marina, Škapin Srečo, Trovatti Eliane, Mančić Lidija
Department of Health and Biological Sciences,Universidade de Araraquara-UNIARA, CEP, Araraquara 14801-340, Brazil.
Institute of Technical Sciences of SASA, P.O. Box 377, 11000 Belgrade, Serbia.
Life (Basel). 2022 Dec 31;13(1):116. doi: 10.3390/life13010116.
Hydroxyapatite attracts great attention as hard tissues implant material for bones and teeth. Its application in reconstructive medicine depends on its biocompatibility, which is in a function of composition and surface properties. The insertion of a protein element in the composition of implants can improve the cell adhesion and the osseointegration. Having this in mind, the proposal of this work was to develop L-alanine-grafted hydroxyapatite nanoparticles and to study their biocompatibility. Two L-alanine sources and three grafting methods were used for hydroxyapatite surface functionalization. The efficiency of grafting was determined based on X-ray powder diffraction, Fourier-transform infrared spectroscopy, thermal analyses, and field-emission scanning electron microscopy. The results indicated the formation of hydroxyapatite with 8-25 wt% of organic content, depending on the grafting method. Protein adsorption, cell adhesion, and viability studies were carried out to evaluate biological properties of grafted materials. The viability of MG-63 human osteoblastic cells following 24 h incubation with the alanine-grafted hydroxyapatite samples is well preserved, being in all cases above the viability of cells incubated with hydroxyapatite. The alanine-grafted hydroxyapatite prepared in situ and by simple mixture showed higher protein adsorption and cell adhesion, respectively, indicating their potential toward use in regenerative medicine.
羟基磷灰石作为用于骨骼和牙齿的硬组织植入材料备受关注。其在重建医学中的应用取决于其生物相容性，而生物相容性是组成和表面性质的函数。在植入物组成中引入蛋白质元素可改善细胞黏附及骨整合。基于此，本研究的目的是开发L-丙氨酸接枝的羟基磷灰石纳米颗粒并研究其生物相容性。使用两种L-丙氨酸来源和三种接枝方法对羟基磷灰石进行表面功能化。基于X射线粉末衍射、傅里叶变换红外光谱、热分析和场发射扫描电子显微镜确定接枝效率。结果表明，根据接枝方法的不同，形成了有机含量为8-25 wt%的羟基磷灰石。进行了蛋白质吸附、细胞黏附和活力研究以评估接枝材料的生物学特性。与丙氨酸接枝的羟基磷灰石样品孵育24小时后，MG-63人成骨细胞的活力得到良好保存，在所有情况下均高于与羟基磷灰石孵育的细胞的活力。原位制备和通过简单混合制备的丙氨酸接枝的羟基磷灰石分别表现出更高的蛋白质吸附和细胞黏附，表明它们在再生医学中的应用潜力。
