School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China; School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150090, China.
School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150090, China.
Mater Sci Eng C Mater Biol Appl. 2018 Sep 1;90:706-712. doi: 10.1016/j.msec.2018.04.026. Epub 2018 Apr 14.
Hydroxyapatite (HAP) was very attractive for using as bone implant material for a long period due to the close similarity with natural bone in composition and osteoconductive properties. In this study, three kinds of natural HAP (nHAP) derived from rainbow trout (Onchorynchus mkiss), cod (Gadus) and salmon (Oncorhynchus keta) bones were prepared using thermal calcination method for the first time. Resultant nHAPs were characterized by fourier transform infrared spectroscopy (FT-IR), x-ray diffraction analysis (XRD), field-emission scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) analysis. Biocompatibility of calcined nHAP was evaluated through MTT cell viability assay and alkaline phosphatase activity experiment using mouse preosteoblast MC3T3-E1. Results of cell experiment indicated that the nHAP originated from rainbow trout and salmon bones showed better biological compatibility compared with the nHAP originated from cod bone and chemical synthetic HAP (cHAP). This is most likely attributed to the different element composition in nHAP, i.e., the nHAP derived from rainbow trout and salmon bones showed the presence of CO and Mg. Therefore, the nHAP originated from rainbow trout and salmon bones have a great potential for application as implant material substitute in bone tissue engineering and the natural waste fish bone product can be used for hydroxyapatite synthesis as a part of bio-waste management.
羟基磷灰石（HAP）由于其在组成和骨传导性方面与天然骨非常相似，因此在很长一段时间内都非常吸引人，可作为骨植入材料使用。在这项研究中，首次使用热煅烧法从虹鳟鱼（Onchorynchus mkiss）、鳕鱼（Gadus）和三文鱼（Oncorhynchus keta）骨骼中制备了三种天然 HAP（nHAP）。通过傅里叶变换红外光谱（FT-IR）、X 射线衍射分析（XRD）、场发射扫描电子显微镜（SEM）和能谱分析（EDS）对所得 nHAP 进行了表征。通过 MTT 细胞活力测定和碱性磷酸酶活性实验，评估了煅烧 nHAP 的生物相容性，使用的细胞为小鼠前成骨细胞 MC3T3-E1。细胞实验结果表明，与来源于鳕鱼骨骼的 nHAP 相比，来源于虹鳟鱼和三文鱼骨骼的 nHAP 显示出更好的生物相容性。这很可能归因于 nHAP 中不同的元素组成，即来源于虹鳟鱼和三文鱼骨骼的 nHAP 中存在 CO 和 Mg。因此，来源于虹鳟鱼和三文鱼骨骼的 nHAP 具有作为骨组织工程中植入材料替代品的巨大潜力，天然废弃鱼骨产品可作为生物废物管理的一部分用于合成羟基磷灰石。
