Kim Jinhyuk, Bae Hyuncheol, Han Hyuk-Soo, Lee Jungwoo
Department of Electronic Engineering, Kwangwoon University, Seoul 01897, Republic of Korea.
Department of Orthopedic Surgery, Seoul National University College of Medicine, Seoul 03080, Republic of Korea.
Micromachines (Basel). 2023 Jan 13;14(1):202. doi: 10.3390/mi14010202.
We recently investigated the design and fabrication of Langevin-type transducers for therapeutic ultrasound. Effect of ultrasonic energy arising from the transducer on biological tissue was examined. In this study, the transducer was set to radiate acoustic energy to mesenchymal stem cells (MSCs) for inducing differentiation into cartilage tissue. The average chondrogenic ratio in area was 20.82% in the control group, for which no external stimulation was given. Shear stress was applied to MSCs as the contrast group, which resulted in 42.66% on average with a 25.92% minimum rate; acoustic pressure from the flat tip causing transient cavitation enhanced chondrogenesis up to 52.96%. For the round tip excited by 20 V, the maximum differentiation value of 69.43% was found, since it delivered relatively high acoustic pressure to MSCs. Hence, the results from this study indicate that ultrasound pressure at the kPa level can enhance MSC chondrogenesis compared to the tens of kHz range by Langevin transducers.
我们最近研究了用于治疗性超声的兰姆波型换能器的设计与制造。研究了换能器产生的超声能量对生物组织的影响。在本研究中，将换能器设置为向间充质干细胞（MSCs）辐射声能，以诱导其分化为软骨组织。对照组未给予外部刺激，其平均软骨生成面积比为20.82%。作为对比组，对MSCs施加剪切应力，平均产生42.66%的软骨生成，最低发生率为25.92%；扁平尖端产生的导致瞬态空化的声压使软骨生成增强至52.96%。对于由20 V激发的圆形尖端，由于其向MSCs传递了相对较高的声压，发现最大分化值为69.43%。因此，本研究结果表明，与兰姆波换能器在几十千赫兹范围内相比，千帕级的超声压力可增强MSCs的软骨生成。
