Senevirathne Amal, Lee Jehee, De Zoysa Mahanama, Nikapitiya Chamilani
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.
Fish Vaccine Research Center, Jeju National University, Jeju 63243, Republic of Korea.
Microorganisms. 2023 Jan 13;11(1):207. doi: 10.3390/microorganisms11010207.
Multidrug-resistant members of the complex have become a threat to human lives and animals, including aquatic animals, owing to the limited choice of antimicrobial treatments. Bacteriophages are effective natural tools available to fight against multidrug-resistant bacteria. The bacteriophage KPP-1 was found to be strictly lytic against , a multidrug-resistant isolate, producing clear plaques. The genome sequence analysis of KPP-1 revealed that it comprised 143,369 base pairs with 47% overall GC content. A total of 272 genes (forward 161, complementary 111) encode for 17 tRNAs and 255 open reading frames (ORFs). Among them, 32 ORFs could be functionally annotated using the National Center for Biotechnology Information (NCBI) Protein Basic Local Alignment Search Tool (BLASTp) algorithm while 223 were found to code for hypothetical proteins. Comparative genomic analysis revealed that the closest neighbor of KPP-1 can be found in the genus of the subfamily . KPP-1 not only markedly suppressed the growth of the host but also worked synergistically with ampicillin. Useful genes for pathogen control such as endolysin (locus tag: KPP_11591) were found to have activity against multidrug-resistant isolate of . Further studies are necessary to develop a strategy to control the emerging pathogen using bacteriophages such as KPP-1.
由于抗菌治疗的选择有限，该菌属的多重耐药成员已对包括水生动物在内的人类和动物的生命构成威胁。噬菌体是对抗多重耐药细菌的有效天然工具。噬菌体KPP - 1被发现对一种多重耐药分离株具有严格的裂解性，能产生清晰的噬菌斑。KPP - 1的基因组序列分析表明，它由143,369个碱基对组成，总体GC含量为47%。共有272个基因（正向161个，互补111个）编码17个tRNA和255个开放阅读框（ORF）。其中，32个ORF可使用美国国立生物技术信息中心（NCBI）的蛋白质基本局部比对搜索工具（BLASTp）算法进行功能注释，而223个被发现编码假设蛋白。比较基因组分析表明，KPP - 1的最接近亲缘种可在该亚科的属中找到。KPP - 1不仅显著抑制宿主生长，还与氨苄西林协同作用。发现诸如内溶素（基因座标签：KPP_11591）等用于病原体控制的有用基因对的多重耐药分离株具有活性。有必要进一步开展研究，以制定利用诸如KPP - 1等噬菌体控制新兴病原体的策略。
