Department of Nutrition and Food Hygiene, Key Laboratory of Environmental Medicine Engineering of Ministry of Education, School of Public Healthy, Southeast University, Nanjing 210009, China.
College of Biology and Food Engineering, Technology Research Center of Characteristic Biological Resources in Northeast of Chongqing, Chongqing Three Gorges University, Chongqing 404000, China.
Molecules. 2023 Jan 6;28(2):579. doi: 10.3390/molecules28020579.
Chrysanthemum extract possesses antioxidant potential and carbohydrate and fat digestive enzyme inhibitory in vitro. However, no evidence supporting chrysanthemum in modulation of postprandial lipemia and antioxidant status in humans presently exists. This study was to analyze the composition of Imperial Chrysanthemum (IC) extract and determine the effect on changes in postprandial glycemic and lipemic response and antioxidant status in adults after consumption of a high-fat (HF) meal. UHPLC-MS method was used to analyze the components of two kinds of IC extracts (IC-P/IC-E) and in vitro antioxidant activities were evaluated using 1,1-diphenyl-2-picrylhydraxyl (DPPH), 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and Hydroxyl radical (HR) radical scavenging assays. Following a randomized design, 37 healthy adults (age, 25.2 ± 2.6 years, and BMI, 20.9 ± 1.5 kg/m2) were assigned to two groups that consumed the HF meal, or HF meal supplemented by IC extract. Blood samples were collected at fasting state and then at 0.5, 1, 2, 4, 6 and 8 h after the meal consumption. There were 12 compounds with relative content of more than 1% of the extracts, of which amino acid and derivatives, flavonoids, carboxylic acids and derivatives were the main components. Compared with IC-E, the contents of flavonoids in IC-P increased significantly (p < 0.05), and the cynaroside content exceeded 30%. In addition, IC-P showed strong free radical scavenging activity against DPPH, ABTS and HR radicals. Furthermore, according to repeated−measures ANOVA, significant differences were observed in the maximal changes for postprandial glucose, TG, T-AOC and MDA among the two groups. Postprandial glucose has significant difference between the two groups at 1 h after meal and the level in IC group was significantly lower than that in control group. No significant differences were observed in the incremental area under the curve (iAUC) among the two groups. IC significantly improved the serum antioxidant status, as characterized by increased postprandial serum T-AOC, SOD, GSH and decreased MDA. This finding suggests that IC can be used as a natural ingredient for reducing postprandial lipemia and improving the antioxidant status after consuming a HF meal.
菊花提取物具有抗氧化潜力和体外抑制碳水化合物和脂肪消化酶的作用。然而，目前没有证据支持菊花能调节人体餐后血脂和抗氧化状态。本研究旨在分析皇菊（IC）提取物的成分，并确定其对高脂（HF）餐后餐后血糖和血脂反应以及抗氧化状态变化的影响。采用 UHPLC-MS 法分析两种 IC 提取物（IC-P/IC-E）的成分，并采用 1,1-二苯基-2-苦基肼（DPPH）、2,2-连氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）和羟基自由基（HR）清除试验评估体外抗氧化活性。采用随机设计，将 37 名健康成年人（年龄 25.2±2.6 岁，BMI 20.9±1.5kg/m2）分为两组，分别摄入 HF 餐或 HF 餐加 IC 提取物。在空腹状态下和餐后 0.5、1、2、4、6 和 8 小时采集血样。提取物中有 12 种化合物的相对含量超过 1%，其中氨基酸及其衍生物、类黄酮、羧酸及其衍生物是主要成分。与 IC-E 相比，IC-P 中的类黄酮含量显著增加（p＜0.05），且金合欢苷含量超过 30%。此外，IC-P 对 DPPH、ABTS 和 HR 自由基具有很强的自由基清除活性。此外，根据重复测量方差分析，两组间餐后血糖、TG、T-AOC 和 MDA 的最大变化差异有统计学意义。餐后 1 小时，两组间餐后血糖差异有统计学意义，IC 组明显低于对照组。两组间曲线下面积增量（iAUC）差异无统计学意义。IC 显著改善了血清抗氧化状态，表现为餐后血清 T-AOC、SOD、GSH 增加，MDA 降低。这一发现表明，IC 可作为一种天然成分，用于减少高脂餐后血脂，并改善高脂餐后的抗氧化状态。
