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不同菊花茶中多糖的结构表征、抗氧化活性及抗糖化活性

Structural characterization, antioxidant activity, and antiglycation activity of polysaccharides from different chrysanthemum teas.

作者信息

Yuan Qin, Fu Yuan, Xiang Pan-Yin, Zhao Li, Wang Sheng-Peng, Zhang Qing, Liu Yun-Tao, Qin Wen, Li De-Qiang, Wu Ding-Tao

机构信息

College of Food Science, Sichuan Agricultural University Ya'an 625014 Sichuan China

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau Macao China.

出版信息

RSC Adv. 2019 Nov 1;9(61):35443-35451. doi: 10.1039/c9ra05820f. eCollection 2019 Oct 31.

Abstract

Polysaccharides are one of the major bioactive components in chrysanthemum teas. In order to understand well the chemical structures and bioactivities of polysaccharides from different chrysanthemum teas (JHPs) collected in China, the physicochemical characteristics, antioxidant activity, and antiglycation activity of polysaccharides extracted from different chrysanthemum teas, including , , 'Huangju', 'Gongju', and 'Hangbaiju', were investigated. The results showed that the contents of total uronic acids and total phenolics in JHPs ranged from (28.4 ± 0.3)% to (36.2 ± 0.2)%, and from 9.4 ± 0.7 to 70.2 ± 1.7 mg GAE per g, respectively. The molecular weights of fraction 1 and fraction 2 in JHPs ranged from 4.29 × 10 to 5.88 × 10 Da, and from 4.11 × 10 to 5.24 × 10 Da, respectively. The dominant constituent monosaccharides of JHPs were galacturonic acid, arabinose, and galactose. Furthermore, JHPs, especially polysaccharides extracted from , exerted remarkable ABTS, DPPH, nitric oxide, and hydroxyl radical scavenging activities, as well as strong antiglycation activities. The results are helpful for better understanding of the chemical structures and bioactivities of JHPs, and JHPs may have good potential applications in the functional-food industry.

摘要

多糖是菊花茶中的主要生物活性成分之一。为了深入了解从中国采集的不同菊花茶(JHPs)中多糖的化学结构和生物活性,对从不同菊花茶中提取的多糖的理化特性、抗氧化活性和抗糖化活性进行了研究,这些菊花茶包括、、“黄菊”、“贡菊”和“杭白菊”。结果表明,JHPs中总糖醛酸和总酚的含量分别为(28.4±0.3)%至(36.2±0.2)%,以及每克9.4±0.7至70.2±1.7毫克没食子酸当量。JHPs中组分1和组分2的分子量分别为4.29×10至5.88×10道尔顿,以及4.11×10至5.24×10道尔顿。JHPs的主要单糖成分是半乳糖醛酸、阿拉伯糖和半乳糖。此外,JHPs,尤其是从中提取的多糖,具有显著的ABTS、DPPH、一氧化氮和羟基自由基清除活性,以及较强的抗糖化活性。这些结果有助于更好地了解JHPs的化学结构和生物活性,并且JHPs在功能性食品工业中可能具有良好的潜在应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96ef/9074740/bddc2578ae85/c9ra05820f-f1.jpg

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