College of Pharmacy, Hebei Medical University, Shijiazhuang 050000, China.
College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang 050000, China.
Molecules. 2023 Jan 9;28(2):671. doi: 10.3390/molecules28020671.
Here, a QuEChERS (quick, easy, cheap, effective, rugged, and safe) pretreatment method was combined with UPLC-MS/MS to facilitate the rapid and reliable simultaneous detection of five calcium channel blockers (CCBs) in human plasma. For this approach, samples were treated with 1 mL of acetonitrile, 350 mg of magnesium sulfate, and 70 mg of PSA adsorbent prior to centrifugation. Supernatants then underwent gradient elution for 8 min with an Agilent C18 column using an acetonitrile-water solution supplemented with 5 mmol⋅L of ammonium acetate. This technique exhibited a good linear response in the 1-800 ng⋅mL range for the analyzed drugs, with an R≥ 0.9921, an accuracy of 87.54-113.05%, a matrix effect (ME) of 91.21-116.39%, a precision of 0.19-11.64%, and stability of no more than 10.05%. This time-saving QuEChERS reagent-based pretreatment technique thus allowed for the simultaneous and accurate detection of five CCBs in human plasma samples, providing a promising new basis for therapeutic drug monitoring in patients with hypertension.
这里，一种 QuEChERS（快速、简单、廉价、有效、耐用、安全）预处理方法与 UPLC-MS/MS 相结合，便于快速可靠地同时检测人血浆中的五种钙通道阻滞剂（CCB）。对于这种方法，样品用 1 毫升乙腈、350 毫克硫酸镁和 70 毫克 PSA 吸附剂处理，然后离心。上清液然后用 Agilent C18 柱进行梯度洗脱，使用乙腈-水溶液，补充 5 mmol·L 的乙酸铵，8 分钟即可完成。该技术在分析药物的 1-800 ng·mL 范围内表现出良好的线性响应，R≥0.9921，准确度为 87.54-113.05%，基质效应（ME）为 91.21-116.39%，精密度为 0.19-11.64%，稳定性不超过 10.05%。这种节省时间的基于 QuEChERS 试剂的预处理技术因此允许同时准确地检测人血浆样品中的五种 CCB，为高血压患者的治疗药物监测提供了有前途的新基础。
