Dong Gaoquan, Fan Mengwei, Wang Hainan, Leng Yadong, Sun Junting, Huang Jun, Zhang Hao, Yan Jie
College of Life Sciences, Shihezi University, Shihezi 832003, China.
Institute of Gardening and Greening, Xinjiang Academy of Forestry Sciences, Urumqi 830000, China.
Plants (Basel). 2023 Jan 5;12(2):252. doi: 10.3390/plants12020252.
is a model species for studying natural rubber biosynthesis because its root can produce high-quality rubber. Small rubber particle protein (SRPP), a stress-related gene to multiple stress responses, involves in natural rubber biosynthesis. To investigate the transcriptional regulation of the promoter, the full-length promoter PR0 (2188 bp) and its four deletion derivatives, PR1 (1592 bp), PR2 (1274 bp), PR3 (934 bp), and PR4 (450 bp), were fused to β-glucuronidase (GUS) reporter gene and transformed into tobacco. The GUS tissue staining showed that the five promoters distinctly regulated GUS expression utilizing transient transformation of tobacco. The GUS activity driven by a PR0 promoter was detected in transgenic tobacco leaves, stem and roots, suggesting that the promoter was not tissue-specific. Deletion analyses in transgenic tobacco have demonstrated that the PR3 from -934 bp to -450 bp core region responded strongly to the hormones, methyl jasmonate (MeJA), abscisic acid (ABA), and salicylic acid (SA), and also to injury induction. The gene was highly expressed under hormones and wound-induced conditions. This study reveals the regulation pattern of the promoter, and provides valuable information for studying natural rubber biosynthesis under hormones and wounding stress.
由于其根部能产生高质量的橡胶，是研究天然橡胶生物合成的模式物种。小橡胶粒子蛋白（SRPP）是一种与多种胁迫反应相关的胁迫相关基因，参与天然橡胶的生物合成。为了研究该启动子的转录调控，将全长启动子PR0（2188 bp）及其四个缺失衍生物PR1（1592 bp）、PR2（1274 bp）、PR3（934 bp）和PR4（450 bp）与β-葡萄糖醛酸酶（GUS）报告基因融合，并转化到烟草中。GUS组织染色表明，利用烟草的瞬时转化，这五个启动子明显调控GUS表达。在转基因烟草的叶片、茎和根中检测到由PR0启动子驱动的GUS活性，表明该启动子不是组织特异性的。转基因烟草中的缺失分析表明，从-934 bp到-450 bp的核心区域PR3对激素茉莉酸甲酯（MeJA）、脱落酸（ABA）和水杨酸（SA）以及损伤诱导有强烈反应。该基因在激素和伤口诱导条件下高度表达。本研究揭示了该启动子的调控模式，并为研究激素和伤口胁迫下的天然橡胶生物合成提供了有价值的信息。
