Mehdi-Sefiani Hanaa, Perez-Puyana Víctor, Ostos Francisco José, Sepúlveda Ranier, Romero Alberto, Rafii-El-Idrissi Benhnia Mohammed, Chicardi Ernesto
Department of Engineering and Materials Science and Transportation, University of Seville, 41012 Seville, Spain.
Department of Chemical Engineering, Faculty of Chemistry, University of Seville, 41012 Seville, Spain.
Polymers (Basel). 2023 Jan 5;15(2):275. doi: 10.3390/polym15020275.
A gelatin-based hydrogel was infiltrated and degraded-released in two different titanium foams with porosities of 30 and 60 vol.% (Ti30 and Ti60 foams) and fabricated by the space holder technique to evaluate its potential to act as an innovative, alternative, and localised method to introduce both active pharmaceutical ingredients, such as antibiotics and non-steroidal anti-inflammatory drugs, and growth factors, such as morphogens, required after bone-tissue replacement surgeries. In addition, the kinetic behaviour was studied for both infiltration and degradation-release processes. A higher infiltration rate was observed in the Ti60 foam. The maximum infiltration hydrogel was achieved for the Ti30 and Ti60 foams after 120 min and 75 min, respectively. Further, both processes followed a Lucas-Washburn theoretical behaviour, typical for the infiltration of a fluid by capillarity in porous channels. Regarding the subsequent degradation-release process, both systems showed similar exponential degradation performance, with the full release from Ti60 foam (80 min), versus 45 min for Ti30, due to the greater interconnected porosity open to the surface of the Ti60 foam in comparison with the Ti30 foam. In addition, the optimal biocompatibility of the hydrogel was confirmed, with the total absence of cytotoxicity and the promotion of cell growth in the fibroblast cells evaluated.
一种基于明胶的水凝胶被渗透到两种孔隙率分别为30%和60%（体积分数）的不同钛泡沫（Ti30和Ti60泡沫）中，并在其中进行降解释放。这两种钛泡沫采用空间保持技术制造，旨在评估该水凝胶作为一种创新、替代且局部化的方法，引入骨组织置换手术后所需的活性药物成分（如抗生素和非甾体抗炎药）以及生长因子（如形态发生素）的潜力。此外，还研究了渗透和降解释放过程的动力学行为。在Ti60泡沫中观察到更高的渗透率。Ti30和Ti60泡沫分别在120分钟和75分钟后达到最大渗透水凝胶量。此外，这两个过程均遵循卢卡斯 - 沃什伯恩理论行为，这是流体在多孔通道中通过毛细作用渗透的典型行为。关于随后的降解释放过程，由于Ti60泡沫与Ti30泡沫相比，其与表面相连的孔隙率更大，两个系统均表现出相似的指数降解性能，Ti60泡沫完全释放需80分钟，而Ti30泡沫为45分钟。此外，该水凝胶的最佳生物相容性得到证实，在所评估的成纤维细胞中完全没有细胞毒性且促进了细胞生长。
