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针对 SARS-CoV-2 刺突蛋白的单克隆抗体，适用于当前关注的多种变体的多种应用。

Monoclonal Antibodies Specific for SARS-CoV-2 Spike Protein Suitable for Multiple Applications for Current Variants of Concern.

机构信息

School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia.

Inflammation Biology, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, Australia.

出版信息

Viruses. 2022 Dec 31;15(1):139. doi: 10.3390/v15010139.

DOI:10.3390/v15010139
PMID:36680179
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9863740/
Abstract

The global coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spawned an ongoing demand for new research reagents and interventions. Herein we describe a panel of monoclonal antibodies raised against SARS-CoV-2. One antibody showed excellent utility for immunohistochemistry, clearly staining infected cells in formalin-fixed and paraffin embedded lungs and brains of mice infected with the original and the omicron variants of SARS-CoV-2. We demonstrate the reactivity to multiple variants of concern using ELISAs and describe the use of the antibodies in indirect immunofluorescence assays, Western blots, and rapid antigen tests. Finally, we illustrate the ability of two antibodies to reduce significantly viral tissue titers in K18-hACE2 transgenic mice infected with the original and an omicron isolate of SARS-CoV-2.

摘要

由严重急性呼吸系统综合征冠状病毒 2（SARS-CoV-2）引起的全球 2019 年冠状病毒病（COVID-19）大流行催生了对新的研究试剂和干预措施的持续需求。在此，我们描述了一组针对 SARS-CoV-2 的单克隆抗体。一种抗体在免疫组织化学方面表现出优异的应用价值，可清晰地标记感染了 SARS-CoV-2 原始株和奥密克戎变异株的感染小鼠的福尔马林固定和石蜡包埋肺和脑组织中的感染细胞。我们使用 ELISA 来证明对多种关注变体的反应性，并描述了这些抗体在间接免疫荧光测定、Western blot 和快速抗原检测中的应用。最后，我们说明了两种抗体在感染了 SARS-CoV-2 原始株和奥密克戎分离株的 K18-hACE2 转基因小鼠中显著降低病毒组织滴度的能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59c7/9863740/59ee93c51687/viruses-15-00139-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59c7/9863740/d89aefe073b5/viruses-15-00139-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59c7/9863740/a93b655809fb/viruses-15-00139-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59c7/9863740/0620844c146c/viruses-15-00139-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59c7/9863740/b9e7c4af9648/viruses-15-00139-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59c7/9863740/166c83f670bc/viruses-15-00139-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59c7/9863740/59ee93c51687/viruses-15-00139-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59c7/9863740/d89aefe073b5/viruses-15-00139-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59c7/9863740/a93b655809fb/viruses-15-00139-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59c7/9863740/0620844c146c/viruses-15-00139-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59c7/9863740/b9e7c4af9648/viruses-15-00139-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59c7/9863740/166c83f670bc/viruses-15-00139-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59c7/9863740/59ee93c51687/viruses-15-00139-g006.jpg

相似文献

[1]
Monoclonal Antibodies Specific for SARS-CoV-2 Spike Protein Suitable for Multiple Applications for Current Variants of Concern.

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[2]
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[6]
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[7]
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[8]
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[9]
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[10]
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引用本文的文献

[1]
Tracking inflammation resolution signatures in lungs after SARS-CoV-2 omicron BA.1 infection of K18-hACE2 mice.

PLoS One. 2024

[2]
The effects of iron deficient and high iron diets on SARS-CoV-2 lung infection and disease.

Front Microbiol. 2024-9-4

[3]
Immunobiology of COVID-19: Mechanistic and therapeutic insights from animal models.

Zool Res. 2024-7-18

[4]
Microplastics dysregulate innate immunity in the SARS-CoV-2 infected lung.

Front Immunol. 2024

[5]
SARS-CoV-2 omicron BA.5 and XBB variants have increased neurotropic potential over BA.1 in K18-hACE2 mice and human brain organoids.

Front Microbiol. 2023-11-23

[6]
Rapid inactivation and sample preparation for SARS-CoV-2 PCR-based diagnostics using TNA-Cifer Reagent E.

Front Microbiol. 2023-10-6

[7]
Monovalent Omicron COVID-19 vaccine triggers superior neutralizing antibody responses against Omicron subvariants than Delta and Omicron bivalent vaccine.

Hum Vaccin Immunother. 2023-8

[8]
Ronapreve (REGN-CoV; casirivimab and imdevimab) reduces the viral burden and alters the pulmonary response to the SARS-CoV-2 Delta variant (B.1.617.2) in K18-hACE2 mice using an experimental design reflective of a treatment use case.

bioRxiv. 2023-3-9

本文引用的文献

[1]
Structural basis of broad SARS-CoV-2 cross-neutralization by affinity-matured public antibodies.

Cell Rep Med. 2024-6-18

[2]
Mouse models of COVID-19 recapitulate inflammatory pathways rather than gene expression.

PLoS Pathog. 2022-9

[3]
Preclinical assessment and randomized Phase I study of CT-P63, a broadly neutralizing antibody targeting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

Emerg Microbes Infect. 2022-12

[4]
Replicating RNA platform enables rapid response to the SARS-CoV-2 Omicron variant and elicits enhanced protection in naïve hamsters compared to ancestral vaccine.

EBioMedicine. 2022-9

[5]
SARS-CoV-2 Omicron sublineages exhibit distinct antibody escape patterns.

Cell Host Microbe. 2022-9-14

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Evolution of ACE2-independent SARS-CoV-2 infection and mouse adaption after passage in cells expressing human and mouse ACE2.

Virus Evol. 2022-7-27

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Skin-patch delivered subunit vaccine induces broadly neutralising antibodies against SARS-CoV-2 variants of concern.

Vaccine. 2022-8-12

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Cochrane Database Syst Rev. 2022-7-22

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Monoclonal antibody therapies against SARS-CoV-2.

Lancet Infect Dis. 2022-11

[10]
Resilience of S309 and AZD7442 monoclonal antibody treatments against infection by SARS-CoV-2 Omicron lineage strains.

Nat Commun. 2022-7-2

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