Wang Shuang, Wu Zichen, Wang Yankai, Hong Huilei, Zhang Lijie, Chen Zhaoyang, Zhang Pengkang, Chen Zirui, Zhang Weibo, Zheng Shunli, Li Quanli, Li Wei, Li Xiangyang, Qiu Hua, Chen Jialong
Key Laboratory of Oral Diseases Research of Anhui Province, Stomatologic Hospital and College, Anhui Medical University, Hefei, Anhui 230032, China.
Regen Biomater. 2022 Oct 20;10:rbac082. doi: 10.1093/rb/rbac082. eCollection 2023.
Silver has been widely used for surface modification to prevent implant-associated infections. However, the inherent cytotoxicity of silver greatly limited the scope of its clinical applications. The construction of surfaces with both good antibacterial properties and favorable cytocompatibility still remains a challenge. In this study, a structurally homogeneous dopamine-silver (DA/Ag) nanocomposite was fabricated on the implant surface to balance the antibacterial activity and cytocompatibility of the implant. The results show that the DA/Ag nanocomposites prepared under the acidic conditions (pH = 4) on the titanium surface are homogeneous with higher Ag content, while an obvious core (AgNPs)-shell (PDA) structure is formed under neutral (pH = 7) and alkaline conditions (pH = 10), and the subsequent heat treatment enhanced the stability of PDA-AgNPs nanocomposite coatings on porous titanium. The antibacterial test, cytotoxicity test, hypodermic implantation and osteogenesis test revealed that the homogeneous PDA-AgNPs nanocomposite coating achieved the balance between the antibacterial ability and cytocompatibility, and had the best outcomes for soft tissue healing and bone formation around the implants. This study provides a facile strategy for preparing silver-loaded surfaces with both good antibacterial effect and favorable cytocompatibility, which is expected to further improve the therapeutic efficacy of silver composite-coated dental implants.
银已被广泛用于表面改性以预防植入物相关感染。然而，银固有的细胞毒性极大地限制了其临床应用范围。构建具有良好抗菌性能和良好细胞相容性的表面仍然是一个挑战。在本研究中，在植入物表面制备了结构均匀的多巴胺-银（DA/Ag）纳米复合材料，以平衡植入物的抗菌活性和细胞相容性。结果表明，在钛表面酸性条件（pH = 4）下制备的DA/Ag纳米复合材料均匀且银含量较高，而在中性（pH = 7）和碱性条件（pH = 10）下形成明显的核（AgNPs）-壳（PDA）结构，随后的热处理增强了PDA-AgNPs纳米复合涂层在多孔钛上的稳定性。抗菌测试、细胞毒性测试、皮下植入和骨生成测试表明，均匀的PDA-AgNPs纳米复合涂层实现了抗菌能力和细胞相容性之间的平衡，并且在植入物周围的软组织愈合和骨形成方面具有最佳效果。本研究提供了一种简便的策略来制备具有良好抗菌效果和良好细胞相容性的载银表面，有望进一步提高银复合涂层牙科植入物的治疗效果。
