Odatsu Tetsurou, Kuroshima Shinichiro, Sato Mika, Takase Kazuma, Valanezhad Alireza, Naito Mariko, Sawase Takashi
Department of Applied Prosthodontics, Institute of Biomedical Sciences, Nagasaki University, 1-7-1, Sakamoto, Nagasaki 852-8588, Japan.
Division of Dental Hygiene, Nagasaki University Hospital, 1-7-1, Sakamoto, Nagasaki 852-8588, Japan.
Antibiotics (Basel). 2020 Jun 19;9(6):347. doi: 10.3390/antibiotics9060347.
Peri-implantitis is an inflammatory disease with a relevant focus on the long-term success of dental implants and implant-supported prostheses. The present study focuses on the antibacterial effect of the silver nanoparticle and investigated the suppression of dental plaque adhesion on implant abutment and/or superstructure by micro-wave assistant nanosilver coating in vivo and in vitro. Nanosilver coating on pure titanium was prepared by microwave-assisted synthesis, and characterized by scanning electron microscopy and energy-dispersive X-ray spectroscopy. In vitro studies were conducted to analyze biocompatibility using MTS assay and fluorescence microscopy with human gingival fibroblasts to evaluate antibacterial activity. During the in vivo study, nanosilver coating was applied to the healing abutments, and the prevention of plaque accumulation on nanosilver coating was confirmed by a split-mouth randomized clinical trial. The aggregation of nano-sized particles was found on the titanium surface with an antibacterial effect. The coating had no cytotoxic effect on human gingival fibroblasts. The result of the clinical trial showed that the coating suppressed the dental plaque adhesion on the healing abutments. Nanosilver coating is a promising material with antibacterial properties and can be used for implant abutments and prostheses for preventing peri-implantitis.
种植体周围炎是一种炎症性疾病，对牙种植体和种植体支持的修复体的长期成功具有重要影响。本研究聚焦于银纳米颗粒的抗菌作用，并研究了微波辅助纳米银涂层在体内和体外对种植体基台和/或上部结构上牙菌斑黏附的抑制作用。通过微波辅助合成法在纯钛上制备纳米银涂层，并通过扫描电子显微镜和能量色散X射线光谱进行表征。体外研究采用MTS法分析生物相容性，并利用荧光显微镜观察人牙龈成纤维细胞以评估抗菌活性。在体内研究中，将纳米银涂层应用于愈合基台，并通过一项双盲随机临床试验证实了纳米银涂层对牙菌斑积聚的预防作用。在钛表面发现了具有抗菌作用的纳米级颗粒聚集。该涂层对人牙龈成纤维细胞无细胞毒性作用。临床试验结果表明，该涂层可抑制愈合基台上的牙菌斑黏附。纳米银涂层是一种具有抗菌性能的有前途的材料，可用于种植体基台和修复体以预防种植体周围炎。
