Hines William C, Hines William C
Sandia National Laboratory, Albuquerque, NM, United States.
Department of Biochemistry and Molecular Biology, University of New Mexico School of Medicine, University of NM, Albuquerque, NM, United States.
Front Cell Dev Biol. 2023 Jan 5;10:1080265. doi: 10.3389/fcell.2022.1080265. eCollection 2022.
The application of retroviral vectors in the laboratory requires considerations that often go overlooked but are often easy to circumvent. Here, we discuss the relationship between the observed transduction efficiency of a cell population and per-cell viral insertions-and describe how differential cell-type susceptibilities can confound results. We consider the math underlying this problem and review an alternative approach to the commonly used "multiplicity of infection" (MOI) method of titering and using viral vectors in the biomedical research laboratory.
在实验室中应用逆转录病毒载体需要考虑一些常常被忽视但通常易于规避的因素。在这里，我们讨论细胞群体的观察到的转导效率与每个细胞的病毒插入之间的关系，并描述不同细胞类型的敏感性如何混淆结果。我们考虑这个问题背后的数学原理，并回顾一种替代方法，以替代生物医学研究实验室中常用的滴定和使用病毒载体的“感染复数”（MOI）方法。
