Seo Sung Eun, Ryu Eunsu, Kim Jinyeong, Shin Chan Jae, Kwon Oh Seok
Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, South Korea.
Department of Biotechnology, University of Science & Technology (UST), Daejeon 34141, South Korea.
Sens Actuators B Chem. 2023 Apr 15;381:133364. doi: 10.1016/j.snb.2023.133364. Epub 2023 Jan 13.
Since December 2019, the rapid and sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a priority for public health. Although the lateral flow assay (LFA) sensor has emerged as a rapid and on-site SARS-CoV-2 detection technique, the conventional approach of using gold nanoparticles for the signaling probe had limitations in increasing the sensitivity of the sensor. Herein, our newly suggested methodology to improve the performance of the LFA system could amplify the sensor signal with a facile fabrication method by concentrating fluorescent organic molecules. A large Stokes shift fluorophore (single benzene) was encapsulated into polystyrene nanobeads to enhance the fluorescence intensity of the probe for LFA sensor, which was detected on the test line with a longpass filter under ultraviolet light irradiation. This approach provides comparatively high sensitivity with the limit of detection of 1 ng mL for the SARS-CoV-2 spike protein and a fast detection process, which takes less than 20 min. Furthermore, our sensor showed higher performance than gold nanoparticle-based commercial rapid diagnostics test kits in clinical tests, proving that this approach is more suitable and reliable for the sensitive and rapid detection of viruses, bacteria, and other hazardous materials.
自2019年12月以来，严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的快速灵敏检测已成为公共卫生的优先事项。尽管侧向流动分析（LFA）传感器已成为一种快速的现场SARS-CoV-2检测技术，但使用金纳米颗粒作为信号探针的传统方法在提高传感器灵敏度方面存在局限性。在此，我们新提出的提高LFA系统性能的方法可以通过浓缩荧光有机分子，采用简便的制造方法来放大传感器信号。将大斯托克斯位移荧光团（单苯）封装到聚苯乙烯纳米珠中，以增强LFA传感器探针的荧光强度，该探针在紫外光照射下用长通滤光片在测试线上进行检测。这种方法具有相对较高的灵敏度，对SARS-CoV-2刺突蛋白的检测限为1 ng/mL，且检测过程快速，耗时不到20分钟。此外，我们的传感器在临床试验中表现出比基于金纳米颗粒的商业快速诊断测试试剂盒更高的性能，证明这种方法更适合且可靠地用于病毒、细菌和其他有害物质的灵敏快速检测。
