Li Yiran, Yang Yamin, Li Weitao, Chen Chaofan, Lin Qiao, Huang Haipeng, Gu Yueqing, Jin Xiaofei, Qian Zhiyu
Department of Biomedical Engineering, College of Automation Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 211106, China.
Department of Biomedical Engineering, College of Engineering, China Pharmaceutical University, Nanjing 211198, China.
Biomed Opt Express. 2024 May 16;15(6):3770-3782. doi: 10.1364/BOE.523179. eCollection 2024 Jun 1.
This paper presents the development of a fiber-optic-based fluorescence detection system for multi-scale monitoring of drug distribution in living animals. The integrated system utilized dual laser sources at the wavelengths of 488 nm and 650 nm and three photomultiplier channels for multi-color fluorescence detection. The emission spectra of fluorescent substances were tracked using the time-resolved fluorescence spectroscopy module to continuously monitor their blood kinetics. The fiber bundle, consisting of 30,000 optic filaments, was designed for wide-field mesoscopic imaging of the drug's interactions within organs. The inclusion of a gradient refractive index (GRIN) lens within the setup enabled fluorescence confocal laser scanning microscopy to visualize the drug distribution at the cellular level. The system performance was verified by imaging hepatic and renal tissues in mice using cadmium telluride quantum dots (CdTe QDs) and R3. By acquiring multi-level images and real-time data, our integrated system underscores its potential as a potent tool for drug assessment, specifically within the realms of pharmacokinetic and pharmacodynamic investigations.
本文介绍了一种基于光纤的荧光检测系统的开发，用于对活体动物体内药物分布进行多尺度监测。该集成系统利用波长为488 nm和650 nm的双激光源以及三个光电倍增管通道进行多色荧光检测。使用时间分辨荧光光谱模块跟踪荧光物质的发射光谱，以连续监测其血液动力学。由30000根光纤丝组成的光纤束被设计用于对药物在器官内的相互作用进行宽视野介观成像。装置中包含的梯度折射率（GRIN）透镜使荧光共聚焦激光扫描显微镜能够在细胞水平上可视化药物分布。通过使用碲化镉量子点（CdTe QDs）和R3对小鼠肝脏和肾脏组织进行成像，验证了该系统的性能。通过获取多层次图像和实时数据，我们的集成系统突出了其作为药物评估有力工具的潜力，特别是在药代动力学和药效学研究领域。
