State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, PR China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, 430070, PR China.
College of Science, Huazhong Agricultural University, Wuhan, 430070, China.
Talanta. 2021 Apr 1;225:122064. doi: 10.1016/j.talanta.2020.122064. Epub 2021 Jan 2.
Quantum dots (QDs) based fluorescent nanobeads are considered as promising materials for next generation point-of-care diagnosis systems. In this study, we carried out, for the first time, the synthesis of QDs nanobeads using polystyrene (PS) nanobead as the template. QDs loading on PS nanobead surface in this method can be readily achieved by the use of polyelectrolyte, avoiding the time-consuming and uncontrollable silane reagents-involved functionalization procedure that conventional synthesis of silica-based QDs nanobeads often suffer from. Notably, the application of QDs nanobeads in suspension microarray for H5N1 virus detection leads to a sensitivity lower than 25 PFU/mL. In addition, QDs nanobead was also incorporated into lateral flow assay for SARS-CoV-2 antibody detection, leading to more than one order of magnitude detection sensitivity as compared to that of commercial one based on colloid gold.
基于量子点（QDs）的荧光纳米珠被认为是下一代即时诊断系统有前途的材料。在这项研究中，我们首次使用聚苯乙烯（PS）纳米珠作为模板合成了 QDs 纳米珠。在这种方法中，通过使用聚电解质可以很容易地将 QDs 负载到 PS 纳米珠表面，避免了传统合成基于二氧化硅的 QDs 纳米珠时经常遇到的耗时且不可控的硅烷试剂官能化过程。值得注意的是，将 QDs 纳米珠应用于悬浮微阵列中进行 H5N1 病毒检测，其灵敏度低于 25 PFU/mL。此外，QDs 纳米珠还被整合到侧向流动测定法中用于 SARS-CoV-2 抗体检测，与基于胶体金的商业试剂盒相比，灵敏度提高了一个数量级以上。
