Islam Sajedul, Bhowmik Dipty Rani, Roy Sourav, Rahman Shuvo Md Sadikur, Begum Rahima, Hasan Maruful, Amin Mohammad Tohidul, Ud Daula Afm Shahid, Hossain Mohammad Salim
Department of Pharmacy, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh.
Department of Microbiology, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh.
Heliyon. 2022 Dec 30;9(1):e12737. doi: 10.1016/j.heliyon.2022.e12737. eCollection 2023 Jan.
Obesity is a severe public health burden and a major component of metabolic syndrome. It is critical to identify treatment medicines for obesity and associated inflammation. We examined the anti-obesity and anti-inflammatory properties of seeds methanol extract in high-fat diet-induced obesity. Changes in body weight, Lee index, fat mass accumulation, serum cholesterol, and serum triglyceride were monitored. Alteration in the expression of PPARγ, GLUT4, and MCP-1 at the transcript level in adipose tissue was also studied. After tabulation of our data, a significant reduction (p < 0.05) was recorded for body weight gain, and fat mass accumulation followed by significant changes (p < 0.05) in serum cholesterol, and serum triglyceride levels by the extract. In agreement with the biochemical data, the extract was capable enough (p < 0.05) to reduce the mRNA expression of PPARγ, and MCP-1, confirming the ability of the extract to ameliorate the risk of obesity and obesity-associated inflammation. Moreover, an in-silico study showed the high binding affinity of the reported compounds from like Delphinidin, Umbelliferon with COX-2, PPARγ, and MCP-1, supporting the notion of the risk-reducing potential of for obesity and obesity mediated inflammatory.
肥胖是一项严重的公共卫生负担，也是代谢综合征的主要组成部分。确定肥胖及相关炎症的治疗药物至关重要。我们研究了[种子名称]甲醇提取物在高脂饮食诱导的肥胖中的抗肥胖和抗炎特性。监测了体重、李氏指数、脂肪量积累、血清胆固醇和血清甘油三酯的变化。还研究了脂肪组织中转录水平上PPARγ、GLUT4和MCP-1表达的改变。在整理我们的数据后，提取物使体重增加和脂肪量积累显著减少（p < 0.05），随后血清胆固醇和血清甘油三酯水平也发生了显著变化（p < 0.05）。与生化数据一致，提取物有足够能力（p < 0.05）降低PPARγ和MCP-1的mRNA表达，证实了提取物改善肥胖风险及肥胖相关炎症的能力。此外，一项计算机模拟研究表明，所报道的化合物如飞燕草素、伞形花内酯与COX-2、PPARγ和MCP-1具有高结合亲和力，支持了[种子名称]对肥胖及肥胖介导的炎症具有降低风险潜力的观点。
