National Influenza Center, National Institute of Hygiene and Epidemiology, Hanoi, Viet Nam.
Department of Infection Control, 108 Military Central Hospital, Hanoi, Viet Nam.
Western Pac Surveill Response J. 2022 Sep 19;13(3):1-5. doi: 10.5365/wpsar.2022.13.3.955. eCollection 2022 Jul-Sep.
The Omicron variant caused a surge of infections with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Viet Nam in early 2022, signalling community transmission. We report on active whole-genome sequencing surveillance of positive SARS-CoV-2 samples collected at that time in northern Viet Nam from international arrivals and community clusters. We used an amplicon protocol developed with 14 polymerase chain reaction products and the Illumina iSeq 100 platform. Overall, 213 nasopharyngeal or throat swabs were analysed, of which 172 samples were identified with the Omicron variant. Of these, 80 samples were collected from community cases in February 2022, among which 59 samples were sublineage BA.2 and one sample was the recombinant XE variant. Our results indicated that Omicron had replaced Delta as the dominant variant in a very short period of time and that continuously conducting active whole-genome sequencing surveillance is necessary in monitoring the evolution and genomic diversity of SARS-CoV-2 in Viet Nam.
2022 年初，奥密克戎变异株导致越南新冠病毒 2（SARS-CoV-2）感染病例激增，标志着社区传播的开始。我们报告了对当时在越南北部从国际入境者和社区集群中采集的阳性 SARS-CoV-2 样本进行的主动全基因组测序监测。我们使用了一种带有 14 个聚合酶链反应产物的扩增子方案和 Illumina iSeq 100 平台。总共分析了 213 份鼻咽或咽喉拭子，其中 172 份样本鉴定为奥密克戎变异株。其中，80 份样本来自 2022 年 2 月的社区病例，其中 59 份样本为亚谱系 BA.2，1 份样本为重组 XE 变异株。我们的结果表明，奥密克戎在很短的时间内取代了德尔塔，成为主要变异株，因此在越南有必要持续进行主动全基因组测序监测，以监测 SARS-CoV-2 的进化和基因组多样性。
