CAS Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; Key Laboratory of Low-carbon Green Agriculture in Tropical region of China, Ministry of Agriculture and Rural Affairs, Hainan Key Laboratory of Tropical Eco-Circular Agriculture, Environmental and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; Hainan Danzhou Tropical Agro-ecosystem National Observation and Research Station, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China; University of Chinese Academy of Sciences, Beijing 100049, China.
CAS Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Sci Total Environ. 2023 Apr 15;869:161742. doi: 10.1016/j.scitotenv.2023.161742. Epub 2023 Jan 21.
Antibiotic resistance gene (ARG)-contaminated food from manure application is gaining widespread interest, but little is known about the distribution and uptake of ARGs in peanuts that are subjected to manure routinely. In this study, the ARG profile and bacterial community in soil and peanut plants from a 7-year manure-fertilized field were investigated using high-throughput qPCR and 16S rRNA gene sequencing. Manure application increased the abundance of ARGs in soil and peanuts by 59-72 and 4-10 fold, respectively. The abundance of ARGs from high to low was as follows: manure, shell-sphere soil, rhizosphere soil, bulk soil, stems, shells, needles, kernels, and roots. Source-tracker analyses were used to investigate the potential source of ARGs in peanut kernels, which revealed that the ARGs in peanut kernels may be primarily absorbed by the roots from the soil. The horizontal gene transfer (HGT) of ARGs was the primary factor in the spread of ARGs, and Proteobacteria were the primary agents of HGT between different parts of peanut plants. Additionally, norank_Chloroplast from the phylum Cyanobacteria was the most important contributor to the abundance of ARGs in peanut kernels. Overall, our findings fill a gap in our understanding of the distribution patterns of ARGs in peanut plants and the migratory pathways of ARGs from soil to peanut kernels.
受粪便施入影响的抗生素耐药基因（ARG）污染食物正受到广泛关注，但对于经常接受粪便施肥的花生中 ARG 的分布和摄取情况知之甚少。在本研究中，采用高通量 qPCR 和 16S rRNA 基因测序技术，研究了经过 7 年粪肥施肥的农田中土壤和花生植物中的 ARG 图谱和细菌群落。粪便施入使土壤和花生中的 ARG 丰度分别增加了 59-72 倍和 4-10 倍。ARGs 的丰度从高到低依次为：粪肥、壳-球土壤、根际土壤、原状土壤、茎、壳、针、仁、根。源追踪分析用于研究花生仁中 ARGs 的潜在来源，结果表明，花生仁中的 ARGs 可能主要通过根系从土壤中吸收。水平基因转移（HGT）是 ARGs 传播的主要因素，变形菌门是花生植物不同部位之间 HGT 的主要媒介。此外，蓝藻门的未分类_叶绿体是花生仁中 ARGs 丰度的最重要贡献者。总的来说，我们的研究结果填补了花生植物中 ARGs 分布模式以及 ARGs 从土壤向花生仁迁移途径的研究空白。
