Department of Pharmacy, Banasthali Vidyapith, Banasthali, Rajasthan, India.
Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, Raebareli, Lucknow, Uttar Pradesh, India.
J Ethnopharmacol. 2023 Apr 24;306:116206. doi: 10.1016/j.jep.2023.116206. Epub 2023 Jan 21.
In the Ayurvedic system of medicine, Cedrus deodara bark has been utilized as a folk medicine to remove ovarian cysts and treat infertility in females.
The present study is the first to investigate ameliorating potential of C. deodara bark on testosterone propionate and high-fat diet-induced polycystic ovarian syndrome in experimental rats.
LC-MS analysis of the fraction selected through bioassay-guided approach employing uterine relaxant activity was performed to determine the bioactive constituents present in it. Further, the identified compounds were docked on the catalytic site of the androgen receptor and insulin receptor substrate-1. Later, the fraction was investigated against testosterone propionate and high-fat diet-induced PCOS in rats.
Chloroform fraction (F1) of the plant bark was found most active in uterine smooth muscle relaxant activity. LC-MS analysis of F1 indicated the presence of key flavonoids namely deodarin, cedrin, deodardione, and cedrusinin. Afterward, a molecular docking study of these compounds revealed impressive binding interactions with androgen receptor and insulin receptor substrate-1. Besides, in vivo studies, treatment with F1 significantly restored the estrous cycle in rats from the diestrus phase in a dose-dependent manner. Also, the disturbed metabolic and endocrine profile was markedly improved in rats. Later, histopathological analysis revealed the presence of a large number of mature follicles and corpora lutea in F1-treated rats.
In a nutshell, F1 exhibited promising beneficial effects in PCOS and associated conditions via amelioration of metabolic, endocrine, and ovarian dynamics in experimental rats.
在印度阿育吠陀医学体系中，雪松皮被用作民间药物，用于去除卵巢囊肿和治疗女性不孕。
本研究首次调查了雪松皮对丙酸睾酮和高脂肪饮食诱导的多囊卵巢综合征实验大鼠的改善潜力。
采用子宫松弛活性的生物测定指导方法对所选部分进行 LC-MS 分析，以确定其中存在的生物活性成分。此外，鉴定出的化合物被对接在雄激素受体和胰岛素受体底物-1 的催化部位上。随后，该部分被用于研究丙酸睾酮和高脂肪饮食诱导的 PCOS 大鼠。
植物皮的氯仿部分（F1）在子宫平滑肌松弛活性方面最为活跃。F1 的 LC-MS 分析表明存在关键的黄酮类化合物，即去氧松脂素、雪松脂素、去氧松脂酮和雪松因。随后，这些化合物的分子对接研究表明与雄激素受体和胰岛素受体底物-1 具有令人印象深刻的结合相互作用。此外，在体内研究中，F1 以剂量依赖的方式显著恢复了大鼠从动情前期到动情期的发情周期。此外，大鼠的代谢和内分泌紊乱谱也得到了显著改善。随后，组织病理学分析显示 F1 治疗的大鼠卵巢中存在大量成熟卵泡和黄体。
总之，F1 通过改善实验大鼠的代谢、内分泌和卵巢动力学，对 PCOS 及其相关病症表现出有希望的有益作用。
