Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Switzerland.
Euro Surveill. 2022 Dec;27(49). doi: 10.2807/1560-7917.ES.2022.27.49.2200343.
IntroductionMeat can be a vehicle for food-borne transmission of antimicrobial resistant bacteria and antimicrobial resistance genes. The occurrence of extended-spectrum beta-lactamase (ESBL) producing Enterobacterales has been observed in meat from livestock production but has not been well studied in meat from wild game.AimWe aimed to investigate, particularly in central Europe, to what extent ESBL-producing Enterobacterales may be present in wild game meat.MethodsA total of 111 samples of different types of game meat supplied by butchers, hunters, retail stores and a large game-processing establishment in Europe were screened for ESBL-producing Enterobacterales using a selective culture medium. Isolates were genotypically and phenotypically characterised.ResultsThirty-nine samples (35% of the total) yielded ESBL-producing Enterobacterales, with most (35/39) supplied by the game-processing establishment. Isolates included 32 , 18 and one . PCR screening identified (n = 31), (n = 8), (n = 4), (n = 3), (n = 1), (n = 1), (n = 1), and (n = 2). Most belonged to phylogenetic group A (n = 7) or B1 (n = 9), but several isolates belonged to extraintestinal pathogenic (ExPEC) sequence types (ST)58 (n = 4), ST68 (n = 1) and ST540 (n = 1). Whole genome sequencing of six selected isolates localised on megaplasmids in four and on IncN_1 plasmids in one and one . Forty-eight isolates (94%) exhibited a multidrug-resistance phenotype.ConclusionWe found a high occurrence of ESBL-producing Enterobacterales in wild game meat, suggesting wildlife habitat pollution and possible microbial contamination events occurring during skinning or cutting carcasses.
引言
肉类可能是食源性传播抗微生物药物耐药细菌和抗微生物药物耐药基因的载体。在牲畜生产的肉类中已经观察到产生超广谱β-内酰胺酶（ESBL）的肠杆菌科的出现，但在野生野味肉类中尚未得到很好的研究。
目的
我们旨在调查，特别是在中欧，产 ESBL 的肠杆菌科在野生野味肉类中可能存在的程度。
方法
总共筛选了来自欧洲屠夫、猎人、零售店和大型野味加工企业的 111 种不同类型的野味肉类样本，使用选择性培养基筛选产 ESBL 的肠杆菌科。对分离株进行了基因和表型特征分析。
结果
39 个样本（占总数的 35%）产生了产 ESBL 的肠杆菌科，其中大部分（35/39）来自野味加工企业。分离株包括 32 株 、18 株 和 1 株 、3 株 、1 株 、1 株 、1 株 、1 株 和 2 株 。PCR 筛选鉴定出 （n = 31）、 （n = 8）、 （n = 4）、 （n = 3）、 （n = 1）、 （n = 1）、 （n = 1）和 （n = 2）。大多数 属于 A 群（n = 7）或 B1 群（n = 9），但有几个分离株属于肠外致病性 （ExPEC）序列型 58（n = 4）、68（n = 1）和 540（n = 1）。对 6 株选定分离株的全基因组测序将 定位在 4 株 中的大型质粒上，将 定位在 1 株 和 1 株 中的 IncN_1 质粒上。48 株（94%）表现出多药耐药表型。
结论
我们发现野生野味肉类中 ESBL 产生肠杆菌科的发生率很高，这表明野生动物栖息地受到污染，在剥皮或切割胴体期间可能发生了微生物污染事件。
