Komatsu Yosuke, Tsuda Muneya, Wada Yasuaki, Shibasaki Takuya, Nakamura Hirohiko, Miyaji Kazuhiro
Health Care & Nutritional Science Institute, Morinaga Milk Industry Co. Ltd., Zama252-8583, Japan.
Center for Food and Medical Innovation Promotion, Institute for the Promotion of Business-Regional Collaboration of Hokkaido University, Sapporo001-0021, Japan.
J Agric Food Chem. 2023 Feb 8;71(5):2503-2513. doi: 10.1021/acs.jafc.2c07273. Epub 2023 Jan 25.
The INFOGEST method is a valuable tool for understanding and monitoring food digestion as an alternative to in vivo assays. However, few studies have compared animal and alternative protein sources in terms of protein quality using the INFOGEST method. This study aimed to evaluate the protein quality of milk-, plant-, and insect-based protein materials by in vitro protein digestibility and in vitro digestible indispensable amino acid score (DIAAS), following the INFOGEST method. Milk-based protein materials had the highest protein digestibility (86.1-90.8%), followed by soy (85.1%) and wheat (82.3%). These materials had significantly higher protein digestibility compared with zein (65.1%), cricket (63.6%), and mealworm (69.5%). Additionally, the mean values of in vitro DIAAS of milk-based protein materials (105.0-137.5) were higher than those of plant- and insect-based protein materials (1.9-91.0). Milk-based protein materials have higher protein quality than plant- and insect-based protein materials by the nutritional evaluation following the INFOGEST digestion method.
INFOGEST方法是一种用于理解和监测食物消化的有价值工具，可作为体内试验的替代方法。然而，很少有研究使用INFOGEST方法比较动物蛋白源和替代蛋白源的蛋白质质量。本研究旨在按照INFOGEST方法，通过体外蛋白质消化率和体外可消化必需氨基酸评分（DIAAS）来评估基于牛奶、植物和昆虫的蛋白质材料的蛋白质质量。基于牛奶的蛋白质材料具有最高的蛋白质消化率（86.1-90.8%），其次是大豆（85.1%）和小麦（82.3%）。与玉米醇溶蛋白（65.1%）、蟋蟀（63.6%）和黄粉虫（69.5%）相比，这些材料的蛋白质消化率显著更高。此外，基于牛奶的蛋白质材料的体外DIAAS平均值（105.0-137.5）高于基于植物和昆虫的蛋白质材料（1.9-91.0）。按照INFOGEST消化方法进行营养评估，基于牛奶的蛋白质材料比基于植物和昆虫的蛋白质材料具有更高的蛋白质质量。
