Division of Surgical Oncology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Division of Surgical Oncology, Nagoya University Graduate School of Medicine, Nagoya, Japan
Anticancer Res. 2023 Feb;43(2):621-630. doi: 10.21873/anticanres.16198.
BACKGROUND/AIM: Deep ultraviolet (DUV) light spans within the 250 nm to 350 nm invisible wavelength range. Although it strongly damages various cells, the efficacy of DUV irradiation on pancreatic cancer cells has never been clarified. The purpose of this study was to reveal the antitumor effects of DUV irradiation on pancreatic cancer cells.
Human pancreatic cancer cell lines were eradicated with DUV or ultraviolet A (UVA) for 5 s. Several angiogenesis-related proteins were studied in cancer cells after DUV irradiation using a protein antibody array. A subcutaneous xenograft model was established by inoculation of pancreatic cancer cells into mice. Tumors in this model were irradiated with DUV or UVA once or twice for two weeks. Tumor volumes in these groups (DUV×1: one irradiation, DUV×2: two irradiations, and untreated) were analyzed one week after the second irradiation.
DUV irradiation significantly changed the cytomorphology of pancreatic cancer cells. In addition, DUV irradiation induced apoptosis on pancreatic cancer cells more strongly than UVA irradiation and no irradiation. Interestingly, lower expression of thrombospondin 1 (TSP1) and tissue inhibitor of metalloproteinase 1 (TIMP1) was identified after DUV treatment. The tumor volume in the DUV-treated groups (DUV×1 and DUV×2) was smaller than that in the untreated group.
Further investigations are required to reveal the precise mechanisms of the antitumor effects of DUV irradiation and to facilitate its clinical application as a new therapy for pancreatic cancer. Overall, DUV irradiation can be potentially used as a therapeutic option of pancreatic malignancy.
背景/目的：深紫外线（DUV）光在 250nm 至 350nm 不可见波长范围内。尽管它强烈破坏各种细胞，但 DUV 照射对胰腺癌细胞的疗效尚未明确。本研究旨在揭示 DUV 照射对胰腺癌细胞的抗肿瘤作用。
用 DUV 或紫外线 A（UVA）照射 5s 消灭人胰腺癌细胞系。用蛋白质抗体阵列研究 DUV 照射后癌细胞中几种血管生成相关蛋白。通过将胰腺癌细胞接种到小鼠中建立皮下异种移植模型。用 DUV 或 UVA 对该模型中的肿瘤进行一次或两次照射，共两周。分析照射后两周内这些组（DUV×1：一次照射，DUV×2：两次照射，未处理）的肿瘤体积。
DUV 照射显著改变了胰腺癌细胞的细胞形态。此外，DUV 照射诱导胰腺癌细胞凋亡的作用强于 UVA 照射和无照射。有趣的是，DUV 处理后发现血小板反应蛋白 1（TSP1）和金属蛋白酶组织抑制剂 1（TIMP1）的表达降低。DUV 处理组（DUV×1 和 DUV×2）的肿瘤体积小于未处理组。
需要进一步研究以揭示 DUV 照射抗肿瘤作用的确切机制，并促进其作为胰腺癌新疗法的临床应用。总之，DUV 照射可能是治疗胰腺癌的一种潜在选择。
