Department of Nano Bio Engineering, Seoul National University of Science and Technology, Seoul 01811, Republic of Korea.
Department of Food Science and Technology, Seoul National University of Science and Technology, Seoul 01811, Republic of Korea.
J Microbiol Biotechnol. 2023 Mar 28;33(3):356-362. doi: 10.4014/jmb.2211.11049. Epub 2022 Dec 13.
The 2'-fucosyllactose (2'-FL) is the richest components in a human milk oligosaccharide. Several studies have reported that 2'-FL has beneficial effects in infants. However, there are few studies on its immune-enhancing effects. This research aimed to examine the immune-enhancing effect of 2'-FL on immunosuppression by cyclophosphamide (CCP) in ICR mice. Mice were orally administered distilled water or 0.5 mg/kg B.W. 2'-FL for 14 days. An immunocompromised mouse model was induced using CCP 80 mg/kg B.W. at 12-14 days. Using the CCP had effects on reducing their body weight, organ weight, spleen index, natural killer (NK) cell activity, and cytokines concentration and expression. This study also used concanavalin A-mediated T-cell proliferation to verify the immune-enhancing effects in the sample. Body weight, spleen index, organ weight, and cytokine levels were measured to estimate the immune-enhancing effects. The body weight at 14 days tended to increase, and the spleen weight and index significantly increased in the 2'-FL group compared to the CCP group. The NK cell activity increased in the 2'-FL group compared to the CCP group, but there was no significant difference. The concentration of interleukin (IL)-2 tended to recover in the 2'-FL group compared to the CCP group. The 2'-FL group showed a significant increase of IL-10 and IFN-gamma concentration compared to the CCP group. In addition, there was a trend of increased IL-10 mRNA expression compared to the CCP group. These results revealed that 2'-FL improved CCP-induced immunosuppression, suggesting that 2'-FL may have the potential to enhance the immune system.
2'-岩藻糖基乳糖（2'-FL）是人乳低聚糖中最丰富的成分。多项研究报道 2'-FL 对婴儿有有益作用。然而，关于其免疫增强作用的研究较少。本研究旨在探讨 2'-FL 对环磷酰胺（CCP）诱导的 ICR 小鼠免疫抑制的免疫增强作用。小鼠连续 14 天经口给予蒸馏水或 0.5mg/kg BW 2'-FL。第 12-14 天用 80mg/kg BW CCP 诱导免疫功能低下的小鼠模型。CCP 降低了小鼠体重、器官重量、脾指数、自然杀伤（NK）细胞活性、细胞因子浓度和表达。本研究还使用刀豆蛋白 A 介导的 T 细胞增殖来验证样品中的免疫增强作用。通过测量体重、脾指数、器官重量和细胞因子水平来评估免疫增强作用。与 CCP 组相比，第 14 天的体重有增加的趋势，2'-FL 组的脾重和指数显著增加。与 CCP 组相比，2'-FL 组的 NK 细胞活性增加，但无显著性差异。与 CCP 组相比，2'-FL 组白细胞介素（IL）-2 的浓度有恢复的趋势。与 CCP 组相比，2'-FL 组的 IL-10 和 IFN-γ浓度显著增加。此外，与 CCP 组相比，2'-FL 组的 IL-10 mRNA 表达有增加的趋势。这些结果表明，2'-FL 改善了 CCP 诱导的免疫抑制，提示 2'-FL 可能具有增强免疫系统的潜力。
