Tulliez M, Villeval J L, Lejeune F, Henri A, Testa U, Titeux M, Rochant H, Breton-Gorius J, Vainchenker W
Inserm U. 91, Hôpital Henri Mondor, Creteil, France.
Leukemia. 1987 Jan;1(1):44-51.
The expression of blood group A antigen on marrow and blood cells from A1 and A2 subjects was investigated by the binding of Helix pomatia and Dolichos biflorus lectins using immunofluorescence. These two lectins stained BFU-E-derived colonies from A subjects in the early days of culture before the expression of glycophorin. The erythroid origin of these cells was ascertained by the coexpression of two other very early erythroid markers. In bone marrow, the ultrastructural immunogold method revealed that the entire erythroid lineage including proerythroblasts was labeled by HPA, whereas no staining was observed on granulomonocytic cells including myeloblasts. Platelets from A subjects were HPA-labeled and so were platelets from an O subject preincubated in A plasma. Megakaryocytes obtained in CFU-MK-derived colonies were weakly and heterogeneously labeled by the HPA lectin. Cultures from A1 and A2 subjects were the reflection of the genetic differences only when investigations were performed on mature erythroblasts. In contrast, the great majority of immature erythroblasts both from A2 and A1 subjects were equally labeled by both lectins; during further erythroid maturation, binding of both lectins markedly diminished only on A2 erythroblasts. When marrow erythroblasts were investigated at electron microscopic level, heterogeneity of labeling among all stages of maturation was clearly observed in A2 subjects, with staining stronger on immature than on mature erythroblasts. Therefore, the genetic differences between A1 and A2 subjects are revealed during terminal erythroid differentiation.
采用免疫荧光法，通过苹果蜗牛凝集素和双花扁豆凝集素的结合，研究了A1和A2型个体骨髓及血细胞上A血型抗原的表达情况。在血型糖蛋白表达之前的培养早期，这两种凝集素可对A型个体来源的爆式红系集落形成单位（BFU-E）衍生的集落进行染色。通过另外两种极早期红系标志物的共表达，确定了这些细胞的红系起源。在骨髓中，超微结构免疫金法显示，包括早幼红细胞在内的整个红系谱系均被人血小板同种抗体（HPA）标记，而包括原粒细胞在内的粒单核细胞则未观察到染色。A型个体的血小板被HPA标记，在A型血浆中预孵育的O型个体的血小板也被HPA标记。从CFU-MK衍生集落中获得的巨核细胞被HPA凝集素弱阳性且不均匀地标记。仅在对成熟红细胞进行研究时，A1和A2型个体的培养物才反映出遗传差异。相反，来自A2和A1型个体的绝大多数未成熟红细胞均被两种凝集素同等标记；在红系进一步成熟过程中，两种凝集素的结合仅在A2型红细胞上明显减少。当在电子显微镜水平研究骨髓红细胞时，在A2型个体中清楚地观察到成熟各阶段标记的异质性，未成熟红细胞的染色强于成熟红细胞。因此，A1和A2型个体之间的遗传差异在红系终末分化过程中得以揭示。
