Thermo Fisher Scientific, Materials and Structural Analysis, Achtseweg Noord 5, 5651 GG, Eindhoven, The Netherlands.
AstraZeneca, Mechanistic and Structural Biology, Discovery Sciences, R&D, 431 50, Mölndal, Sweden.
Sci Rep. 2023 Jan 25;13(1):1420. doi: 10.1038/s41598-023-28455-w.
Membrane proteins are the largest group of therapeutic targets in a variety of disease areas and yet, they remain particularly difficult to investigate. We have developed a novel one-step approach for the incorporation of membrane proteins directly from cells into lipid Salipro nanoparticles. Here, with the pannexin1 channel as a case study, we demonstrate the applicability of this method for structure-function analysis using SPR and cryo-EM.
膜蛋白是各种疾病领域中最大的治疗靶点群体，但它们仍然特别难以研究。我们开发了一种从细胞中直接将膜蛋白纳入脂质 Salipro 纳米颗粒的新型一步法。在这里，以 Pannexin1 通道为例，我们展示了该方法在使用 SPR 和 cryo-EM 进行结构功能分析方面的适用性。
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