Wang Yaling, Pan Tiezheng, Wei Xuewen, Su Fangcui, Li Ang, Tai Yifan, Wei Tingting, Zhang Qian, Kong Deling, Zhang Chunqiu
State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials of Ministry of Education and College of Life Sciences, Nankai University, Tianjin, China.
School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan.
Commun Chem. 2022 Jul 9;5(1):81. doi: 10.1038/s42004-022-00700-9.
Enzymatic compartments, inspired by cell compartmentalization, which bring enzymes and substrates together in confined environments, are of particular interest in ensuring the enhanced catalytic efficiency and increased lifetime of encapsulated enzymes. Herein, we constructed bioinspired enzymatic compartments (TPE-Q18H@GPs) with semi-permeability by spatiotemporally controllable self-assembly of catalytic peptide TPE-Q18H in hollow porous glucan particles (GPs), allowing substrates and products to pass in/out freely, while enzymatic aggregations were retained. Due to the enrichment of substrates and synergistic effect of catalytic nanofibers formed in the confined environment, the enzymatic compartments exhibited stronger substrate binding affinity and over two-fold enhancement of second-order kinetic constant (k/K) compared to TPE-Q18H nanofibers in disperse system. Moreover, GPs enabled the compartments sufficient stability against perturbation conditions, such as high temperature and degradation. This work opens an intriguing avenue to construct enzymatic compartments using porous biomass materials and has fundamental implications for constructing artificial organelles and even artificial cells.
受细胞区室化启发的酶促区室，能在受限环境中将酶和底物聚集在一起，在确保提高包封酶的催化效率和延长其寿命方面具有特殊意义。在此，我们通过催化肽TPE-Q18H在中空多孔葡聚糖颗粒（GPs）中的时空可控自组装，构建了具有半渗透性的仿生酶促区室（TPE-Q18H@GPs），使底物和产物能够自由进出，同时保留酶聚集体。由于底物的富集以及在受限环境中形成的催化纳米纤维的协同作用，与分散体系中的TPE-Q18H纳米纤维相比，酶促区室表现出更强的底物结合亲和力和二阶动力学常数（k/K）超过两倍的增强。此外，GPs使区室对诸如高温和降解等扰动条件具有足够的稳定性。这项工作为使用多孔生物质材料构建酶促区室开辟了一条有趣的途径，对构建人工细胞器甚至人工细胞具有重要的基础意义。
