Mecê Pedro, Scholler Jules, Groux Kassandra, Boccara Claude
Institut Langevin, ESPCI Paris, CNRS, PSL University, 1 rue Jussieu, 75005 Paris, France.
Biomed Opt Express. 2019 Dec 23;11(1):492-504. doi: 10.1364/BOE.381398. eCollection 2020 Jan 1.
Time-domain full-field OCT (FF-OCT) represents an imaging modality capable of recording high-speed sections of a sample at a given depth. One of the biggest challenges to transfer this technique to image human retina is the presence of continuous involuntary head and eye axial motion during image acquisition. In this paper, we demonstrate a solution to this problem by implementing an optical stabilization in an FF-OCT system. This was made possible by combining an FF-OCT system, an SD-OCT system, and a high-speed voice-coil translation stage. B-scans generated by the SD-OCT were used to measure the retina axial position and to drive the position of the high-speed voice coil translation stage, where the FF-OCT reference arm is mounted. Closed-loop optical stabilization reduced the RMS error by a factor of 7, significantly increasing the FF-OCT image acquisition efficiency. By these means, we demonstrate the capacity of the FF-OCT to resolve cone mosaic as close as 1.5 from the fovea center with high consistency and without using adaptive optics.
时域全场光学相干断层扫描（FF - OCT）是一种能够在给定深度记录样本高速截面的成像方式。将该技术应用于人体视网膜成像面临的最大挑战之一是在图像采集过程中存在持续的不自主头部和眼球轴向运动。在本文中，我们通过在FF - OCT系统中实施光学稳定化来展示解决此问题的方案。这通过将FF - OCT系统、谱域光学相干断层扫描（SD - OCT）系统和高速音圈平移台相结合得以实现。由SD - OCT生成的B扫描用于测量视网膜轴向位置，并驱动安装有FF - OCT参考臂的高速音圈平移台的位置。闭环光学稳定化将均方根误差降低了7倍，显著提高了FF - OCT图像采集效率。通过这些方法，我们展示了FF - OCT在不使用自适应光学的情况下，能够以高一致性从中央凹中心分辨出间距低至1.5的视锥细胞镶嵌结构的能力。
