碳酸饮料影响小鼠雄激素受体水平及睾酮分泌
CARBONATED BEVERAGES AFFECT LEVELS OF ANDROGEN RECEPTOR AND TESTOSTERONE SECRETION IN MICE.
作者信息
Gong Z, Yuan Z, Niu Y, Zhang X, Geng J, Wei S
机构信息
Northwest Minzu University, Affiliated Hospital, Lanzhou, China.
Northwest Minzu University, Life Science and Engineering College, Lanzhou, China.
出版信息
Acta Endocrinol (Buchar). 2022 Jul-Sep;18(3):301-305. doi: 10.4183/aeb.2022.301.
OBJECTIVES
This work aimed to study the influences of carbonated beverages (CBs) on the testis growth and the expression levels of androgen receptor (AR) of mice.
METHODS
Two experimental groups of 30 mice each PEP-1 and PEP-2 drank 50% and 100% Pepsi-Cola, respectively for 15 days. Other 2 experimental groups of 30 mice each COC-1 and COC-2 drank 50% and 100% Coca-Cola, respectively for 15 days. The control group (CG) of 30 mice drank water. Bilateral testes were harvested aseptically on days 0, 5, 7, 10, 13 and 15. Real-time PCR and Western blot were implemented to detect levels of androgen receptor (AR) mRNA and protein in testis tissues.
RESULTS
Testes masses of PEP-2, COC-1 and COC-2 were greater than those of PEP-1 and CG (P < 0.05). On day 15, testis longitudinal diameter (TLD) of CBs-treated mice was increased as compared to CG. TLD, testes transverse diameters (TTD) and AR proteins levels of PEP-2 and COC-2 were increased in comparison with CG (P<0.05). Serum testosterone concentrations of PEP-2 were higher than that of COC-1 and CG (P < 0.05). Levels of AR mRNAs of four CBs-treated mice were increased by 60.18%, 67.26%, 65.93% and 78.76%.
CONCLUSIONS
A high concentration of Coca-Cola and Pepsi-Cola could raise TLD and TDD, enhance testosterone secretion, and increase serum EGF concentrations.
目的
本研究旨在探讨碳酸饮料（CBs）对小鼠睾丸生长及雄激素受体（AR）表达水平的影响。
方法
将60只小鼠分为两组，每组30只，分别为PEP - 1组和PEP - 2组，分别饮用50%和100%的百事可乐，持续15天。另外两组，每组30只，分别为COC - 1组和COC - 2组，分别饮用50%和100%的可口可乐，持续15天。对照组（CG）的30只小鼠饮用清水。在第0、5、7、10、13和15天无菌采集双侧睾丸。采用实时荧光定量PCR和蛋白质免疫印迹法检测睾丸组织中雄激素受体（AR）mRNA和蛋白水平。
结果
PEP - 2组、COC - 1组和COC - 2组的睾丸重量大于PEP - 1组和CG组（P < 0.05）。在第15天，与CG组相比，饮用碳酸饮料的小鼠睾丸纵径（TLD）增加。与CG组相比，PEP - 2组和COC - 2组的TLD、睾丸横径（TTD）和AR蛋白水平升高（P<0.05）。PEP - 2组的血清睾酮浓度高于COC - 1组和CG组（P < 0.05）。四种饮用碳酸饮料的小鼠AR mRNA水平分别升高了60.18%、67.26%、65.93%和78.76%。
结论
高浓度的可口可乐和百事可乐可增加TLD和TDD，增强睾酮分泌，并提高血清表皮生长因子浓度。
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