Pila Pattiya, Chuammitri Phongsakorn, Patchanee Prapas, Pringproa Kidsadagon, Piyarungsri Kakanang
Department of Companion Animal and Wildlife Clinic, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand.
Research Center of Producing and Development of Products and Innovations for Animal Health and Production, Chiang Mai University, Chiang Mai, Thailand.
Front Vet Sci. 2023 Jan 9;9:1043848. doi: 10.3389/fvets.2022.1043848. eCollection 2022.
Chronic kidney disease (CKD) is a frequent condition in elderly cats. Bcl-2 is linked to kidney disease through the processes of apoptosis and fibrosis. The purpose of this study is to examine Bcl-2 levels in CKD and clinically healthy age-matched cats in order to evaluate the relationship between Bcl-2 levels, signalment, and blood parameters in cats with CKD. The circulating levels of Bcl-2 were determined using an immunoassay in twenty-four CKD cats and eleven clinically healthy age-matched cats by the utilization of the general linear model (GLM), Pearson correlation, principal component analysis (PCA), ROC curves, the Cox hazard model, and Kaplan-Meier survival analysis. These were all conducted in order to explore Bcl-2 levels and their connection with other variables. The Bcl-2 immunohistochemical intensity was graded in each glomerulus and tubulointerstitium. McNemar's test was performed in order to compare the expression of Bcl-2 in the two renal tissue sites. The circulating Bcl-2 of CKD cats was significantly lower than those of clinically healthy age-matched cats ( = 0.034). The presence of circulating Bcl-2 ( < 0.01) and the severity of CKD ( = 0.02) were both linked with the survival time of cats with CKD. The area under the curve (AUC) of Bcl-2 for detection of CKD was 0.723. In cats, decreased circulating Bcl-2 was associated with increased blood BUN, creatinine levels, and CKD severity. Bcl-2 protein expression was reduced in the renal tissues of CKD cats as the disease progressed, resulting in a decrease in their survival time. This study demonstrated that Bcl-2 may be effective in diagnosing feline CKD.
慢性肾脏病（CKD）在老年猫中较为常见。Bcl-2通过细胞凋亡和纤维化过程与肾脏疾病相关联。本研究的目的是检测CKD猫和临床健康的年龄匹配猫的Bcl-2水平，以评估CKD猫的Bcl-2水平、特征和血液参数之间的关系。通过使用一般线性模型（GLM）、Pearson相关性分析、主成分分析（PCA）、ROC曲线、Cox风险模型和Kaplan-Meier生存分析，利用免疫测定法测定了24只CKD猫和11只临床健康的年龄匹配猫的循环Bcl-2水平。所有这些都是为了探索Bcl-2水平及其与其他变量的联系。对每个肾小球和肾小管间质中的Bcl-2免疫组化强度进行分级。进行McNemar检验以比较Bcl-2在两个肾组织部位的表达。CKD猫的循环Bcl-2明显低于临床健康的年龄匹配猫（P = 0.034）。循环Bcl-2的存在（P < 0.01）和CKD的严重程度（P = 0.02）均与CKD猫的生存时间相关。Bcl-2检测CKD的曲线下面积（AUC）为0.723。在猫中，循环Bcl-2降低与血液中尿素氮、肌酐水平升高以及CKD严重程度增加有关。随着疾病进展，CKD猫肾组织中的Bcl-2蛋白表达降低，导致其生存时间缩短。本研究表明，Bcl-2可能对诊断猫CKD有效。
