PoET：测量巨型单层囊泡中孔边缘张力的自动化方法。

PoET: automated approach for measuring pore edge tension in giant unilamellar vesicles.

作者信息

Leomil Fernanda S C, Zoccoler Marcelo, Dimova Rumiana, Riske Karin A

机构信息

Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo 043039-032, Brazil.

Department of Theory and Bio Systems, Max Planck Institute of Colloids and Interfaces, Potsdam 14424, Germany.

出版信息

Bioinform Adv. 2021 Nov 23;1(1):vbab037. doi: 10.1093/bioadv/vbab037. eCollection 2021.

DOI:10.1093/bioadv/vbab037

PMID:36700098

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9710609/

Abstract

MOTIVATION

A reliable characterization of the membrane pore edge tension of single giant unilamellar vesicles (GUVs) requires the measurement of micrometer sized pores in hundreds to thousands of images. When manually performed, this procedure has shown to be extremely time-consuming and to generate inconsistent results among different users and imaging systems. A user-friendly software for such analysis allowing quick processing and generation of reproducible data had not yet been reported.

RESULTS

We have developed a software (PoET) for automatic pore edge tension measurements on GUVs. The required image processing steps and the characterization of the pore dynamics are performed automatically within the software and its use allowed for a 30-fold reduction in the analysis time. We demonstrate the applicability of the software by comparing the pore edge tension of GUVs of different membrane compositions and surface charges. The approach was applied to electroporated GUVs but is applicable to other means of pore formation.