Max Planck Institute of Colloids and Interfaces, Science Park Golm, 14476 Potsdam, Germany; Institute for Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany.
Max Planck Institute of Colloids and Interfaces, Science Park Golm, 14476 Potsdam, Germany.
Biophys J. 2022 Sep 6;121(17):3295-3302. doi: 10.1016/j.bpj.2022.06.011. Epub 2022 Jun 6.
Cell membranes are highly asymmetric and their stability against poration is crucial for survival. We investigated the influence of membrane asymmetry on electroporation of giant unilamellar vesicles with membranes doped with GM1, a ganglioside asymmetrically enriched in the outer leaflet of neuronal cell membranes. Compared with symmetric membranes, the lifetimes of micronsized pores are about an order of magnitude longer suggesting that pores are stabilized by GM1. Internal membrane nanotubes caused by the GM1 asymmetry, obstruct and additionally slow down pore closure, effectively reducing pore edge tension and leading to leaky membranes. Our results point to the drastic effects this ganglioside can have on pore resealing in biotechnology applications based on poration as well as on membrane repair processes.
细胞膜具有高度的不对称性，其抵抗穿孔的稳定性对生存至关重要。我们研究了膜不对称性对用 GM1 掺杂的巨大单层囊泡电穿孔的影响，GM1 是一种在外层叶中不对称富集的神经细胞膜糖脂。与对称膜相比，微米级孔的寿命长约一个数量级，这表明 GM1 稳定了孔。GM1 不对称引起的内部膜纳米管阻塞并进一步减缓孔闭合，有效降低孔边缘张力并导致渗漏膜。我们的结果表明，这种神经节苷脂在基于穿孔的生物技术应用以及膜修复过程中对孔再封闭可能产生重大影响。
