Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea.
FEBS Open Bio. 2023 Mar;13(3):532-544. doi: 10.1002/2211-5463.13564. Epub 2023 Feb 7.
The intestinal epithelium is a single-cell layer on the mucosal surface that absorbs food-derived nutrients and functions as a barrier that protects mucosal integrity. Hesperidin (hesperetin-7-rhamnoglucoside) is a flavanone glycoside composed of the flavanone hesperetin and the disaccharide rutinose, which has various physiological benefits, including antioxidative, anti-inflammatory, and antiallergic effects. Here, we used human intestinal Caco-2 cell monolayers to examine the effect of hesperidin on intestinal barrier function. Hesperidin-treated Caco-2 cell monolayers displayed enhanced intestinal barrier integrity, as indicated by an increase in transepithelial electrical resistance (TEER) and a decreased apparent permeability (P ) for fluorescein. Hesperidin elevated the mRNA and protein levels of occludin, MarvelD3, JAM-1, claudin-1, and claudin-4, which are encoded by tight junction (TJ)-related genes. Moreover, hesperidin significantly increased the phosphorylation of AMP-activated protein kinase (AMPK), indicating improved intestinal barrier function. Thus, our results suggest that hesperidin enhances intestinal barrier function by increasing the expression of TJ-related occludin, MarvelD3, JAM-1, and claudin-1 via AMPK activation in human intestinal Caco-2 cells.
肠道上皮是黏膜表面的单层细胞，可吸收食物来源的营养物质，并作为保护黏膜完整性的屏障发挥作用。橙皮苷（橙皮素-7-鼠李糖苷）是由黄烷酮橙皮素和二糖芦丁组成的黄烷酮糖苷，具有多种生理益处，包括抗氧化、抗炎和抗过敏作用。在这里，我们使用人肠道 Caco-2 细胞单层来研究橙皮苷对肠道屏障功能的影响。橙皮苷处理的 Caco-2 细胞单层显示出增强的肠道屏障完整性，表现为跨上皮电阻（TEER）增加和荧光素的表观通透性（P ）降低。橙皮苷上调了紧密连接（TJ）相关基因编码的闭合蛋白、MarvelD3、JAM-1、Claudin-1 和 Claudin-4 的 mRNA 和蛋白水平。此外，橙皮苷显著增加了 AMP 激活蛋白激酶（AMPK）的磷酸化，表明肠道屏障功能得到改善。因此，我们的结果表明，橙皮苷通过在人肠道 Caco-2 细胞中激活 AMPK 增加 TJ 相关闭合蛋白、MarvelD3、JAM-1 和 Claudin-1 的表达来增强肠道屏障功能。
